中国现代医学杂志
中國現代醫學雜誌
중국현대의학잡지
CHINA JOURNAL OF MODERN MEDICINE
2001年
1期
35-36
,共2页
凋亡%晶状体上皮细胞%紫外光%白内障
凋亡%晶狀體上皮細胞%紫外光%白內障
조망%정상체상피세포%자외광%백내장
目的：研究紫外光(ultraviolet UV)诱导的晶状体上皮细胞(lens epithelial cell LEC)凋亡特征。方法：100mw/m2紫外光照射Vistar大鼠双眼15min，照射后1，6，24h剥取晶状体囊膜。DNA缺口末端原位标记法定量检测凋亡的晶状体上皮细胞并通过透射电镜观察其形态学改变。结果：DNA缺口末端原位标记法检测仅在照射后24h组发生凋亡特染细胞，其百分比为14.3%。透射电镜下可见染色质边集浓缩现象。结论：紫外光照射可快速诱导晶状体上皮细胞凋亡。
目的：研究紫外光(ultraviolet UV)誘導的晶狀體上皮細胞(lens epithelial cell LEC)凋亡特徵。方法：100mw/m2紫外光照射Vistar大鼠雙眼15min，照射後1，6，24h剝取晶狀體囊膜。DNA缺口末耑原位標記法定量檢測凋亡的晶狀體上皮細胞併通過透射電鏡觀察其形態學改變。結果：DNA缺口末耑原位標記法檢測僅在照射後24h組髮生凋亡特染細胞，其百分比為14.3%。透射電鏡下可見染色質邊集濃縮現象。結論：紫外光照射可快速誘導晶狀體上皮細胞凋亡。
목적：연구자외광(ultraviolet UV)유도적정상체상피세포(lens epithelial cell LEC)조망특정。방법：100mw/m2자외광조사Vistar대서쌍안15min，조사후1，6，24h박취정상체낭막。DNA결구말단원위표기법정량검측조망적정상체상피세포병통과투사전경관찰기형태학개변。결과：DNA결구말단원위표기법검측부재조사후24h조발생조망특염세포，기백분비위14.3%。투사전경하가견염색질변집농축현상。결론：자외광조사가쾌속유도정상체상피세포조망。
Objective:To investigate the apoptosis of lens epithelial cell induced by ultraviolet.Methods：Vistar rats received 100mw/m2 ultraviolet irradition(UVR)(λ 280nm-315nm) for 15 minutes.At h1,6,24 after irradition the lens capsules were dissected and the percentage of apoptotic cells were evaluted by the TdT-dUTP terminal nick-end labeling(TUNEL) technique.The morphological changes were observed by transmission electron microscope.Results：TUNEL-positive nuclei were found at only 24 hours after UVR exposure and the percentage is 14.3%.Cell chromatin condensation was observed by transmission electron microscope.Conclusions：Our results showed that UVR can induce the rapid apoptotic death of the lens epithelial cells.
