CAJ | 학술논문

用RT-PCR方法对2例感染新型HEV的样品进行了检测，并对PCR 产物进行了克隆及测序。检测结果显示用常规PCR检测易造成ORF3中GC丰富区的缺失，但在常规PCR反应液中加入GC melt溶液可成功地扩增GC丰富区。序列分析显示T1和T11属于同一基因型，但不同于已报道的HEV I型、II型和III型，为一新的基因型。T1和T11与I型在该区的核苷酸同源性为79 %～82%；与II型的同源性为80%～81%; 和III型的同源性为83%～85%。
용RT-PCR방법대2례감염신형HEV적양품진행료검측，병대PCR 산물진행료극륭급 측서。검측결과현시용상규PCR검측역조성ORF3중GC봉부구적결실，단재상규PCR반응액중가 입GC melt용액가성공지확증GC봉부구。서렬분석현시T1화T11속우동일기인형，단불동우이 보도적HEV I형、II형화III형，위일신적기인형。T1화T11여I형재해구적핵감산동원성위79 %～82%； 여II형적동원성위80%～81%; 화III형적동원성위83%～85%。
ORF3 and partial ORF1 regions were amplified with RT-PCR f rom two patients (T1 and T11)infected with new genotype of hepatitis E Virus. Th e PCR products were cloned and sequenced. The results showed that G-C rich regi on in ORF3 was deleted when amplified with normal PCR reaction. However, PCR rea ction containing G-C melt solution can overcome this problem. The sequence anal ysis showed that T1 and T11 belong to a new genotype of HEV which differs from g enotype I,II and III reported.T1 and T11 have 79%～82%, 80%～81% and 83%～85% id entical to genotype I,II and III respectively.