CAJ | 학술논문

通过对草本植物水双相法分离细胞质膜技术的改进，建立起木本植物泡桐根细胞质膜的分离纯化方法。其改进主要有：在匀浆介质中添加了抗坏血酸，加大了聚乙烯聚吡咯烷酮的用量，由草本植物一般用量0.05%～0.1%增至0.6%；对第1次双相分离后下相液中剩余的质膜进行了再回收。通过对质膜和细胞器各项标记酶指标的测定，确定了6.3%的葡聚糖(Dextra n)T-500和6.3%的聚乙二醇(PEG)4000为主组成的两相系统分离的根细胞质膜具有较高的收率和纯度。该方法的建立为在细胞和分子水平上研究林木根系对离子吸收机理与生长发育的关系打下了基础。
통과대초본식물수쌍상법분리세포질막기술적개진，건립기목본식물포동근세포질막적분리 순화방법。기개진주요유：재균장개질중첨가료항배혈산，가대료취을희취필각완동적용량 ，유초본식물일반용량0.05%～0.1%증지0.6%；대제1차쌍상분리후하상액중잉여적질막 진행료재회수。통과대질막화세포기각항표기매지표적측정，학정료6.3%적포취당(Dextra n)T-500화6.3%적취을이순(PEG)4000위주조성적량상계통분리적근세포질막구유교고적수 솔화순도。해방법적건립위재세포화분자수평상연구림목근계대리자흡수궤리여생장발육적 관계타하료기출。
This paper reports the method of partitioning and purifying plasma membrane from roots of woody plants of Paulownia by improving the method of preparing pla sma membrane by aqueous two-phase partitioning in herbaceous plants. The main i mprovements are adding ascorbic acid and increasing an amount of insoluble pvpp( polyvinylpolypyrrolidone) from 0.05%～0.1% of herbaceous plants to 0.6% in th is homogenization medium, and then again collecting the plasma membrane from the lo wer phase after the first aqueous two-phase partitioning. Through determining t he different kinds of marker enzymes of plasma membranes and organelles, that pl asma membrane preparations of high yield and purity were easily obtained in aque ous polymer two-phase systems mainly constituted by 6.3% Dextran T-500 and 6.3 % polyethylene glycol 4000. This method lays the foundations for the studies on the relation between the principles of roots absorption ions and growth, develop ment of a woody plant at cellular and molecular level.