CAJ | 학술논문

探讨X射线诱导EL-4细胞G1期阻滞及相关蛋白的表达。彩PI荧光标记，流式细胞术检测细胞周期的变化。用单克隆抗体免疫荧光标记，流式细胞术检测蛋白表达的变化。结果表明，2.0Gy和4.0Gy照射后12-72h,G1期EL-4细胞百分数显著高于假照射组（p<0.05-p<0.001）。4.0Gy照射后EL-4细胞p53蛋白表达从照射后2h开始明显增高，持续至照射后24h（p<0.05-p<0.001）;p21蛋白表达在照射后2h开始明显增高，持续至照射后48h（p<0.05-p<0.001）；GADD45蛋白表达在照射后2h开始明显增高，持续至照射后24h（p<0.05-p<0.001）；MDM2蛋白表达在照射后4h开始明显增高，持续至照射后24h（p<0.05-p<0.001）。结果提示，中等剂量X射线照射可诱导EL-4细胞G1期阻滞。p53、p21和GADD45蛋白表达在电离辐射诱导EL-4细胞G1期阻滞中起重要作用
탐토X사선유도EL-4세포G1기조체급상관단백적표체。채PI형광표기，류식세포술검측세포주기적변화。용단극륭항체면역형광표기，류식세포술검측단백표체적변화。결과표명，2.0Gy화4.0Gy조사후12-72h,G1기EL-4세포백분수현저고우가조사조（p<0.05-p<0.001）。4.0Gy조사후EL-4세포p53단백표체종조사후2h개시명현증고，지속지조사후24h（p<0.05-p<0.001）;p21단백표체재조사후2h개시명현증고，지속지조사후48h（p<0.05-p<0.001）；GADD45단백표체재조사후2h개시명현증고，지속지조사후24h（p<0.05-p<0.001）；MDM2단백표체재조사후4h개시명현증고，지속지조사후24h（p<0.05-p<0.001）。결과제시，중등제량X사선조사가유도EL-4세포G1기조체。p53、p21화GADD45단백표체재전리복사유도EL-4세포G1기조체중기중요작용
To investigate G1 arrest and the expressions of relative proteins in EL-4 eclls induced by ionizing radiation,flow cytometry(FCM)was used to analyze cell cyle througyh staining eclls with propidium iodide(PI).And FCM was also employed to analyze protein expression of EL-4 cells stained with monoclonal and polyclonal antibodiss\ for their immunofluorescence.It was found that the number of EL-4 cells in G1 phase increased significantly 12-72h after X-irradiation with doses of 2.0 Gy and 4.0Gy.The results also showed significant increases of p53 protein expression at 2h to 24h(p<0.05-p<0.001),p21 protein expression at 2h to 48h(p<0.05-p<0.001),GADD45 protein expression at 2h to 48h(p<0.05-p<0.001),and MDM2 ptroein expression at 4g to 24h(p<0.05-p<0.001) after 4.0Gy X-irradiation.These results suggest that G1 arrest of EL-4 cells could be induced by ionizing radiation and the expression of p53,p21and GADD45 proteins may play an importatnt role in the mechanism.