莱阳农学院学报
萊暘農學院學報
래양농학원학보
JOURNAL OF LAIYANG AGRICULTURAL COLLEGE
2000年
3期
225-228
,共4页
徐德武%朱永清%杨根海%楚慧民%阎美玉
徐德武%硃永清%楊根海%楚慧民%閻美玉
서덕무%주영청%양근해%초혜민%염미옥
莠去津%放射免疫测定法%水%残留量
莠去津%放射免疫測定法%水%殘留量
유거진%방사면역측정법%수%잔류량
atrazine%radioimmunoassay%water%residue
以莠去津均三氮苯类结构特征,合成连有活性羧基的莠去津半抗原,通过活性酯法制备出具有生物活性的人工抗原,以此免疫兔子获得抗莠去津的多克隆抗体。抗体的特异性强,与扑灭津的交叉反应性为93%,与西玛津的交叉反应性为8%。以3H标记莠去津建立的莠去津放射免疫测定法对水中莠去津添加回收率的测定表明,自来水的添加回收率为88.5%~107.5%,运河水的添加回收率为87.4%~110.9%。
以莠去津均三氮苯類結構特徵,閤成連有活性羧基的莠去津半抗原,通過活性酯法製備齣具有生物活性的人工抗原,以此免疫兔子穫得抗莠去津的多剋隆抗體。抗體的特異性彊,與撲滅津的交扠反應性為93%,與西瑪津的交扠反應性為8%。以3H標記莠去津建立的莠去津放射免疫測定法對水中莠去津添加迴收率的測定錶明,自來水的添加迴收率為88.5%~107.5%,運河水的添加迴收率為87.4%~110.9%。
이유거진균삼담분류결구특정,합성련유활성최기적유거진반항원,통과활성지법제비출구유생물활성적인공항원,이차면역토자획득항유거진적다극륭항체。항체적특이성강,여복멸진적교차반응성위93%,여서마진적교차반응성위8%。이3H표기유거진건립적유거진방사면역측정법대수중유거진첨가회수솔적측정표명,자래수적첨가회수솔위88.5%~107.5%,운하수적첨가회수솔위87.4%~110.9%。
The determination of atrazine residue in water with radioimmunoassay method was studied. The High bioactive antigen was synthesized and the high titer anti-atrazine antibody was prepared. The antiserum was specific for atrazine, the cross-reactivity of the antiserum to propazine and simazine was 93 % and 8 %, respectively. The RIA for atrazine labeled by 3H was established and showed that the rate of recovery by adding atrazine in drink water and yunhe water were 88.5% -107.5 % and 87.4 %-110.9 % respectively.