中国现代应用药学
中國現代應用藥學
중국현대응용약학
CONTENTS IN BRIEF
2003年
1期
42-43
,共2页
加替沙星%高效液相色谱法%荧光检测
加替沙星%高效液相色譜法%熒光檢測
가체사성%고효액상색보법%형광검측
gatifloxacin%HPLC%fluorescence detection
目的建立高效液相色谱荧光法测定加替沙星注射液的含量方法.方法 CLC-ODS柱(15mm×6.0mm, 5μm),流动相:0.05mol/L枸橼酸-乙腈(80∶20),三乙胺调pH3.0,流速1 mL*min-1,Ex=360nm, Em=465nm,样品稀释1000倍,进样10μL.结果加替沙星在4～32ng范围内线性关系良好,回收率(99.98±0.06)%,日内、日间RSD＜2%.结论该法简便、快速、准确,可用于测定加替沙星注射液的含量.
目的建立高效液相色譜熒光法測定加替沙星註射液的含量方法.方法 CLC-ODS柱(15mm×6.0mm, 5μm),流動相:0.05mol/L枸櫞痠-乙腈(80∶20),三乙胺調pH3.0,流速1 mL*min-1,Ex=360nm, Em=465nm,樣品稀釋1000倍,進樣10μL.結果加替沙星在4～32ng範圍內線性關繫良好,迴收率(99.98±0.06)%,日內、日間RSD＜2%.結論該法簡便、快速、準確,可用于測定加替沙星註射液的含量.
목적건립고효액상색보형광법측정가체사성주사액적함량방법.방법 CLC-ODS주(15mm×6.0mm, 5μm),류동상:0.05mol/L구연산-을정(80∶20),삼을알조pH3.0,류속1 mL*min-1,Ex=360nm, Em=465nm,양품희석1000배,진양10μL.결과가체사성재4～32ng범위내선성관계량호,회수솔(99.98±0.06)%,일내、일간RSD＜2%.결론해법간편、쾌속、준학,가용우측정가체사성주사액적함량.
OBJECTIVE To develop an HPLC method with fluorescence detection for analysis of gatifloxacin(GTX) injection.METHOD The HPLC system consisted of CLC-ODS column（15cm×6.0mm, 5μm）and a RF-10AXL fluorescence detector. The mobile phase used composed of 0.05mol*L-1 citric acid-acetonitrile (80∶20, v/v), adjusted pH to 3.0 with triethylamine, and the flow rate was set at 1mL*min-1. The fluorescence detector was set at λex=360nm and λem=465 nm. GTX injection was diluted 1000 times with water, then 10μL was injected into HPLC System. RESULTS The method was linear at the GTX range from 4.0 ng to 32.0ng, the recovery of the method was (99.98±0.06)%(n=5), and the RSD was ＜2% for precision of intra-day and between-day.CONCLUSIONS The method is simple, rapid and accurate, can be used for determination of GTX.
