中国科学B辑(英文版)
中國科學B輯(英文版)
중국과학B집(영문판)
SCIENCE IN CHINA(SCIENTIA SINICA)
2002年
5期
499-454
,共-44页
潘景喜%张素萍%屠铁成%韩镇辉%蔡喜臣%姚思德%林念芸
潘景喜%張素萍%屠鐵成%韓鎮輝%蔡喜臣%姚思德%林唸蕓
반경희%장소평%도철성%한진휘%채희신%요사덕%림념예
actinomycin D%EPR%reactive oxygen species%photodynamic action
Actinomycin D (AMD) is an Received anticancer antibiotic that can bind selectively to both double-stranded and single-stranded DNA, and this binding greatly enhances DNA photosensitization. Using electron paramagnetic resonance (EPR) in combination with spin trapping techniques, a systematic study was carried out on the reactive oxygen species generated in the photosensitization process of AMD. It was found that 1O2 and are important reactive intermediates either in solution or in DNA complexes, and the generation of these species is in competition. This finding suggests that the photodynamic action of AMD proceeds via two pathways: energy transfer (type I mechanism) and electron transfer (type II mechanism). 1O2 is the main product formed via energy transfer reaction in solution while electron transfer between the excited states of AMD and DNA becomes the predominant pathway in DNA complexes.
