CAJ | 학술논문

通过对华北地区耕作制度和生态系统的了解,在充分考虑种群遗传学、生物学和人为操纵因子等三大因素的基础上,建立了一个预测棉铃虫对转Bt基因抗虫棉抗性适应的模拟模型.在华北地区典型的耕作制度下,如果所有棉田均为Bt棉,则Bt棉的预期寿命为7年;如果只有春播棉为Bt棉(约占棉田总面积的70%),则其寿命为10年.模型的灵敏度分析表明,Bt棉的使用寿命随抗性基因的显性度、初始抗性频率、Bt棉所占比例等因素的增长而迅速缩短.当Bt棉表达的杀虫蛋白量恰好全部杀死敏感基因型(GsGs)个体时,Bt棉的预期寿命最短.由于国外采用的"高剂量/庇护所"抗性治理策略不适用于棉铃虫及华北棉区的耕作制度,我国需要加强对其它抗性治理措施(如转双基因抗虫棉)的研究与应用.
통과대화북지구경작제도화생태계통적료해,재충분고필충군유전학、생물학화인위조종인자등삼대인소적기출상,건립료일개예측면령충대전Bt기인항충면항성괄응적모의모형.재화북지구전형적경작제도하,여과소유면전균위Bt면,칙Bt면적예기수명위7년;여과지유춘파면위Bt면(약점면전총면적적70%),칙기수명위10년.모형적령민도분석표명,Bt면적사용수명수항성기인적현성도、초시항성빈솔、Bt면소점비례등인소적증장이신속축단.당Bt면표체적살충단백량흡호전부살사민감기인형(GsGs)개체시,Bt면적예기수명최단.유우국외채용적"고제량/비호소"항성치리책략불괄용우면령충급화북면구적경작제도,아국수요가강대기타항성치리조시(여전쌍기인항충면)적연구여응용.
The commercial use of transgenic cotton expressing an insecticidal protein gene from Bacillus thuringiensis (Bt) began in 1998 in northern China. Resistance management is a major concern for the sustainable use of Bt cotton. With our understanding of the cropping and ecological system in northern China,we developed a simulation model to forecast adaptation of the cotton bollworm,Helicoverpa armigera ables of reproductive fitness on different host plants,dominance of resistance alleles,expression level of Bt toxin in cotton and use of insecticides in Bt cotton fields were included in the model. In the typical cropping system of northern China,the expected life of Bt cotton are seven years if all cotton is Bt cotton,and ten years if only spring planted cotton (about 70% total cotton area) is Bt cotton in northern China besed on the model. The life expectancy decreases quickly with increases in initial frequency of resistance allele,dominance of the resistance gene,and the percent area of Bt cotton planted. The results also showed that supplemental control is essential on Bt cotton when the expression of Bt toxin declines. Because of the lack of a consistent high dose and the complexity of managing individual fields of Bt cotton by many different growers in the same region,the use of the high-dose/refuge strategy in northern China will be difficult to achieve.``