CAJ | 학술논문

In order to understand the biological effect of space environment on growth and development of rice, four pure strains of rice seeds were placed aboard a recoverable satellite system for 15 days, and 12 new lines were selected out after recovery. And their selected progenies were examined for mutations at morphological, physiological and molecular levels, and radiation doses were measured as well. It was found that the seeds received an average dose rate of 0.177 mGy/d and an integral dose of 2.656 mGy/15 days; there were 1.5±0.5 heavy nuclear particles /cm2 of Z/Β≥50(Z≥20) and the numbers of Z≥3 heavy nuclear particles reached 29.7±0.5 particles/cm2 in the satellite cabin; in sum weight of one thousand seeds, 5 lines increased, while 7 lines decreased; in germination power, 7 lines increased, while 5 lines decreased; in germination capacity, 3 lines increased, while 9 lines decreased; in respiration rate of seedling, 6 lines increased, while 5 lines decreased, and one line remained unchanged; in content of chlorophyll, only 1 line increased while 10 lines decreased, and one line remained unchanged. In order to understand better mutagenesis of 971-5 at molecular level, RAPD analysis was conducted with 60 random primers, of which 11.6% were able to generate polymorphic bands between mutated and control plants. It is therefore concluded from the present data that potential uses of crop improvement of rice can be highly mutagenic and can effectively induce some mutations.