CAJ | 학술논문

建立氯霉素,克伦特罗和雌二醇 3种兽药残留同时检测的悬浮芯片法.通过将3种兽药的BSA蛋白结合物偶联于悬浮芯片的固相载体--聚苯乙烯荧光微球上作为检测探针,采用间接竞争法,在液相反应体系中,3种小分子兽药抗原和微球上的兽药结合物共同竞争液相中各自特异性的生物素化单抗,再加入藻红蛋白标记的链霉亲和素,反应后检测获得荧光信号,绘制出3种兽药残留检测的标准曲线.同时进行3种兽药的常规酶联免疫吸附法标准曲线的测定.在检测技术、检出限、检测区间、特异性、盲样测定和多元分析等方面对两种方法进行比较.除了特异性外的其它指标的比较中,悬浮芯片法均具有明显优势.两种方法的特异性检测具有良好的一致性.高通量悬浮芯片技术,具有操作简单、灵敏快速和成本低廉等优点,为多种兽药残留的快速检测提供了新方法.
건립록매소,극륜특라화자이순 3충수약잔류동시검측적현부심편법.통과장3충수약적BSA단백결합물우련우현부심편적고상재체--취분을희형광미구상작위검측탐침,채용간접경쟁법,재액상반응체계중,3충소분자수약항원화미구상적수약결합물공동경쟁액상중각자특이성적생물소화단항,재가입조홍단백표기적련매친화소,반응후검측획득형광신호,회제출3충수약잔류검측적표준곡선.동시진행3충수약적상규매련면역흡부법표준곡선적측정.재검측기술、검출한、검측구간、특이성、맹양측정화다원분석등방면대량충방법진행비교.제료특이성외적기타지표적비교중,현부심편법균구유명현우세.량충방법적특이성검측구유량호적일치성.고통량현부심편기술,구유조작간단、령민쾌속화성본저렴등우점,위다충수약잔류적쾌속검측제공료신방법.
A novel suspension array technology was established for the detection of three kinds of veterinary drug residues: chloramphenicol, clenbuterol and 17-β-estradiol. The three conjugates in which veterinary drugs coupled with BSA were immobilized on the solid carrier of the suspension microarray-polystyrene fluorescent microspheres/beads as detective probes. Indirect competitive technology was employed. Competitive reactions between the veterinary drugs in the aqueous phase and the veterinary drugs-BSA conjugates on the beads for coupling with their complimentary specific biotinylated monoclonal antibodies were carried out. And then, straptavidin-phycoerythrin was added for coupling and the fluorescent signals were captured. Afterwards the detective standard curves were plotted. The regular ELISA standard curves of the three veterinary drugs were also plotted. Comparison between suspension array and regular enzymE-linked immunosorbent assay(ELISA) was in the respects of the detective technology, the detection limits, the detective ranges, the samples detection and the multi-analysis. Suspension array technology is distinct advantageous except for specificity. There was well consistent performance between the two methods. The high-throughput suspension array provides a novel method for multi-analysis of veterinary drugs with simple operation, sensitive, rapid and low costing.