CAJ | 학술논문

利用微波辅助提取枳实总黄酮,并对提取参数进行优化.以过40目筛的脱脂枳实粉为原料,探讨乙醇浓度、液固比、提取时间、微波功率等因素对总黄酮得率及干膏率的影响.在单因素试验基础上,对乙醇浓度、液固比和提取时间3个因素进行Box-Behnken设计,以总黄酮含量、干膏率为响应值,利用响应面分析法对提取参数进行优化.在乙醇浓度70%、液固比40:1(mL/g)、微波功率385W(中火)下提取1.5min,总黄酮含量达到41.39%,干膏率49.00%.响应面法优化枳实黄酮的提取比优化前单因素试验结果有很大的提高.
이용미파보조제취지실총황동,병대제취삼수진행우화.이과40목사적탈지지실분위원료,탐토을순농도、액고비、제취시간、미파공솔등인소대총황동득솔급간고솔적영향.재단인소시험기출상,대을순농도、액고비화제취시간3개인소진행Box-Behnken설계,이총황동함량、간고솔위향응치,이용향응면분석법대제취삼수진행우화.재을순농도70%、액고비40:1(mL/g)、미파공솔385W(중화)하제취1.5min,총황동함량체도41.39%,간고솔49.00%.향응면법우화지실황동적제취비우화전단인소시험결과유흔대적제고.
Studies were made on extracted flavnonids from Frutus aurantii immatures by microwave-assisted extraction, and optimized the extracted factors. The effect of different ethanol concentrations, ratio of solvent to material, extraction time and microwave frequency on yield of total flavonoids and dry extraction ratio were discussed. The Box-Behnken design was applied to fit a response surface function, which based on the one-variable-at-time design. Through the function we can get the optimum extracting conditions, the optimal levels for obtaining 41.39% total flavonoids and 49.0% dry extraction ratio were as follows: The ethanol concentration is 70%, the ratio of solvent to material is 40:1 (mL:g), the microwave frequency is 385 W, and The extraction time is 1.5 min. The Box-Behnken design can be used on the extraction of flavnonids from Frutus aurantii immatures, and the result is better than that in the one-variable-at-time design.