CAJ | 학술논문

目的探讨类风湿关节炎(RA)患者血浆对氧磷酯酶-1(PON-1)活性与氧自由基代谢水平的关系. 方法检测118 例RA患者和56名健康对照者血浆PON-1活性、氧化修饰低密度脂蛋白(ox-LDL)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)和循环谷胱苷肽过氧化物酶(GSH-Px)活性及晚期蛋白质氧化产物(AOPP)水平,分析PON-1活性与氧自由基代谢水平的关系. 结果 RA患者血浆PON-1、SOD、GSH-Px活性分别为(122.2±24.1)kU/L、(78.2±21.3)kU/L 、(156.4±32.2)U/L,低于对照组(P<0.01).ox-LDL、AOPP、MDA水平分别为(832.0±256.2)μg/L、(342.3±118.1) μmol/L、(16.2±6.4) μmol/L,高于对照组(P<0.01).RA患者血浆PON-1活性与GSH-Px、SOD呈正相关(r=0.781,P<0.01;r=0.702,P<0.01),与ox-LDL、MDA、AOPP呈负相关(r=-0.721,P<0.01;r=-0.789,P<0.01;r=-0.679,P<0.01).结论 RA患者血浆PON-1活性降低,PON-1可能通过降低抗氧化能力机制参与了RA的病理生理过程.
목적 탐토류풍습관절염(RA)환자혈장대양린지매-1(PON-1)활성여양자유기대사수평적관계. 방법검측118 례RA환자화56명건강대조자혈장PON-1활성、양화수식저밀도지단백(ox-LDL)、병이철(MDA)함량、초양화물기화매(SOD)화순배곡광감태과양화물매(GSH-Px)활성급만기단백질양화산물(AOPP)수평,분석PON-1활성여양자유기대사수평적관계. 결과 RA환자혈장PON-1、SOD、GSH-Px활성분별위(122.2±24.1)kU/L、(78.2±21.3)kU/L 、(156.4±32.2)U/L,저우대조조(P<0.01).ox-LDL、AOPP、MDA수평분별위(832.0±256.2)μg/L、(342.3±118.1) μmol/L、(16.2±6.4) μmol/L,고우대조조(P<0.01).RA환자혈장PON-1활성여GSH-Px、SOD정정상관(r=0.781,P<0.01;r=0.702,P<0.01),여ox-LDL、MDA、AOPP정부상관(r=-0.721,P<0.01;r=-0.789,P<0.01;r=-0.679,P<0.01).결론 RA환자혈장PON-1활성강저,PON-1가능통과강저항양화능력궤제삼여료RA적병리생리과정.
Objective To investigate the relationship between plasma paraoxonase-1 (PON-1)activity and oxygen free radical (OFR)metabolism in patients with rheumatoid arthritis (RA). Methods The levels of plasma PON-1, oxidized low-density lipoprotein (ox-LDL), malondialdehyde (MDA), superoxide dismutase (SOD), glutathionperoxide (GSH-Px) and advanced oxidation protein products (AOPP) in 118 patients with RA and 56 healthy controls were measured. The correlative relationship between plasma PON-1 activity and OFR metabolism variables was analyzed. Results The levels of plasma PON-1, GSH-Px and SOD in RA patients were(122.2±24.1)kU/L,(156.4±32.2)U/L and (78.2±21.3)kU/L, and these were significantly lower than those in healthy controls (P<0.01). The levels of ox-LDL, MDA and AOPP in RA patients were (832.0±256.2)μg/L,(16.2±6.4) μmol/L and (342.3±118.1) μmol/L, and these were significantly higher than those in healthy controls (P<0.01). PON-1 activity was positively correlated with GSH-Px(r=0.781,P<0.01)and SOD (r=0.702,P<0.01), and it was negatively correlated with ox-LDL (r=-0.721,P<0.01), MDA ( r=-0.789,P<0.01) and AOPP (r=-0.679,P<0.01). Conclusions The plasma PON-1 activity in RA patients decreases significantly. PON-1 may participate in the pathophysiologic process of RA through the reduction of antioxidant properties.