CAJ | 학술논문

叶黄素具有调节人体的免疫能力、防治老年性视黄斑退化和白内障等作用,目前天然叶黄素的提取原料主要为万寿菊花,万寿菊花的有机溶剂提取物含有大量叶黄素酯,经皂化后可转变为叶黄素,广泛用于食品、食品添加剂、药品及饲料等工业.本文采用甲醇处理万寿菊鲜花后直接用正己烷提取叶黄素酯,并通过L9 (34)正交实验选择了叶黄素酯皂化的最佳条件,即KOH/甲醇浓度为20%,提取液﹕KOH为4 : 1,时间为40min,温度为50℃时,叶黄素酯皂化进行的比较完全.皂化得到的叶黄素在丙酮﹕甲醇(v : v)为1 : 1的混合溶剂中进行重结晶,得到叶黄素晶体,纯度为97.2%.
협황소구유조절인체적면역능력、방치노년성시황반퇴화화백내장등작용,목전천연협황소적제취원료주요위만수국화,만수국화적유궤용제제취물함유대량협황소지,경조화후가전변위협황소,엄범용우식품、식품첨가제、약품급사료등공업.본문채용갑순처리만수국선화후직접용정기완제취협황소지,병통과L9 (34)정교실험선택료협황소지조화적최가조건,즉KOH/갑순농도위20%,제취액﹕KOH위4 : 1,시간위40min,온도위50℃시,협황소지조화진행적비교완전.조화득도적협황소재병동﹕갑순(v : v)위1 : 1적혼합용제중진행중결정,득도협황소정체,순도위97.2%.
Lutein can be used to improve human immune system and prevent cataract and senile maculutea degeneration.It can be obtained by saponification of lutein ester,which is the major extracts from Marigold.Lutein is widely used in food,food additive,drugs and feed industries.In this paper,extraction of lutein ester from marigold is conducted with n-hexane as extractant.The best condition for saponifying lutein ester was chosen by the orthogonal experiments of L9 (34).It is found that using 20% KOH methanol as the extracting solution,with saponification reaction time of 40 minutes,at a temperature of 50℃,Lutein ester can be thoroughly sponified.Lutein can then be recrystallized in the solvent of acetone and methanol(v : v 1 : 1).The purity of lutein crystal is 97.2%.