中国法医学杂志
中國法醫學雜誌
중국법의학잡지
CHINESE JOURNAL OF FORENSIC MEDICINE
2009年
6期
392-393,409
,共3页
法医毒物分析%西地那非%HPLC%固相萃取(SPE)%硅胶小柱
法醫毒物分析%西地那非%HPLC%固相萃取(SPE)%硅膠小柱
법의독물분석%서지나비%HPLC%고상췌취(SPE)%규효소주
forensic toxicological analysis%sildenafil%HPLC%SPE%SILICA GEL
目的 建立血中西地那非的HPLC-DAD检测方法.方法 用硅胶小柱分离提取血中的两地那非,HPLC-DAD分析.分析柱:Nova-pak C18(150×3.9mm),5.0μm;保护柱:Phenomenex C18(ODS,4.0×3.0mm,Octadecyl);流动相A:0.06%三氟乙酸+0.06%三乙胺+0.25%乙腈,B:乙腈,梯度程序分离;DAD:230nm.结果 血中西地那非线性检测范围为1.5-80.0μg/mL,最小检出限量为5ng,平均回收率:82.45%.结论 本检验方法简单、准确、快速,适合于临床检测及刑事案件的快速分析.
目的 建立血中西地那非的HPLC-DAD檢測方法.方法 用硅膠小柱分離提取血中的兩地那非,HPLC-DAD分析.分析柱:Nova-pak C18(150×3.9mm),5.0μm;保護柱:Phenomenex C18(ODS,4.0×3.0mm,Octadecyl);流動相A:0.06%三氟乙痠+0.06%三乙胺+0.25%乙腈,B:乙腈,梯度程序分離;DAD:230nm.結果 血中西地那非線性檢測範圍為1.5-80.0μg/mL,最小檢齣限量為5ng,平均迴收率:82.45%.結論 本檢驗方法簡單、準確、快速,適閤于臨床檢測及刑事案件的快速分析.
목적 건립혈중서지나비적HPLC-DAD검측방법.방법 용규효소주분리제취혈중적량지나비,HPLC-DAD분석.분석주:Nova-pak C18(150×3.9mm),5.0μm;보호주:Phenomenex C18(ODS,4.0×3.0mm,Octadecyl);류동상A:0.06%삼불을산+0.06%삼을알+0.25%을정,B:을정,제도정서분리;DAD:230nm.결과 혈중서지나비선성검측범위위1.5-80.0μg/mL,최소검출한량위5ng,평균회수솔:82.45%.결론 본검험방법간단、준학、쾌속,괄합우림상검측급형사안건적쾌속분석.
Objective An analytical approach has been developed for determination of sildenafil blood with high performance liquid chromatography.Methods Sildenafil was extracted by solid phase extraction cartridges from blood and was separated by resered-phase gradient chromatography with DAD detection at 230nm.The analytical column was Nova-pak C18(150×3.9mm),5.0μm and the guard column was Phenomenex C18(ODS,4.0×3.0mm,Octadecyl).The mobile phase Was gradient mixture of acetonitrile(A) and 0.25% acetonitrile aqueous solution including 0.06% trifluoroacetic acid and 0.06% triethylamine (B).Results Linearity Was checked over the concentration range 1.5～80.0μg/mL.Limits of detection was 5.0ng.Average recovery was 82.45%.Conclusion This method is simple.exact and rapid.
