CAJ | 학술논문

目的探讨近交系大鼠肝移植的手术技巧,建立稳定的急性排斥模型.方法 DA大鼠作为供体,雄性Lewis大鼠作为受体,采用改良后的Kamada"二袖套管法",建立大鼠原位肝移植模型.观察术后存活情况,并对其生存时间进行统计分析.结果 60只大鼠肝移植手术中,供体手术时间(30±8)min,修肝时间(15±2)min,受体手术时间(60±7)min,无肝期时间(21±3)min,手术成功率88.3%.术后4～5 d大鼠开始出现黄疸,精神差,睁眼困难,反应迟钝,并于术后11 d内全部死亡.结论根据近交系大鼠自身结构特点,对大鼠原位肝移植模型建立方法进行了改进,取得了一定的成效.DA-Lewis为稳定、可靠的大鼠肝移植急性排斥模型,是研究肝移植排斥反应及免疫耐受的理想动物模型.
목적 탐토근교계대서간이식적수술기교,건립은정적급성배척모형.방법 DA대서작위공체,웅성Lewis대서작위수체,채용개량후적Kamada"이수투관법",건립대서원위간이식모형.관찰술후존활정황,병대기생존시간진행통계분석.결과 60지대서간이식수술중,공체수술시간(30±8)min,수간시간(15±2)min,수체수술시간(60±7)min,무간기시간(21±3)min,수술성공솔88.3%.술후4～5 d대서개시출현황달,정신차,정안곤난,반응지둔,병우술후11 d내전부사망.결론 근거근교계대서자신결구특점,대대서원위간이식모형건립방법진행료개진,취득료일정적성효.DA-Lewis위은정、가고적대서간이식급성배척모형,시연구간이식배척반응급면역내수적이상동물모형.
Objective To establish a steady model of acute rejection of orthotopic liver transplantation (OLT) in inbred rats. Methods With DA rats as donors and inbred Lewis rats as recipients, OLT was performed adopting the improved Kamada "two cuff technique". Results For the 60 rats with orthotopic liver transplantation, the operation time in donors and recipients were (30±8)minutes and (60±7) minutes, respectively. The liver preparation phase in donors was(15±2) minutes and the anhepatic phase in recipients was(21±3)minutes,with a 88.3% successful rate. ALL OLT recipients died within 11 days after operation. Conclusion According to the physiological characteristics, we had achieved some success in improving orthotopic liver transplantion technique in rats. Furthermore, the present model is feasible and enabling comprehensive preclinical experimental research on allograft rejection and tolerance.