化学试剂
化學試劑
화학시제
CHEMICAL REAGENTS
2010年
2期
137-138,182
,共3页
席海山%许良%娜仁花%孙乌日娜
席海山%許良%娜仁花%孫烏日娜
석해산%허량%나인화%손오일나
高效毛细管电泳法%四味土木香散%苦参碱%氧化苦参碱
高效毛細管電泳法%四味土木香散%苦參堿%氧化苦參堿
고효모세관전영법%사미토목향산%고삼감%양화고삼감
high performance capillary electrophoresis (HPCE)%Siweitumuxiang powder%matrine%oxymatrine
提出了同时测定四味土木香散中苦参碱和氧化苦参碱含量的方法.采用高效毛细管电泳法.实验条件为:用缓冲溶液20 mmol/L Tris-40 mmol/L NaH_2PO_4-20%异丙醇(用磷酸调到pH 5.5)作为电泳液,检测波长为200 nm.被测样品中苦参碱和氧化苦参碱完全分离且被准确测定.其平均加样回收率分别为99.2%和100.1%,RSD分别为2.31%和1.57%(n=9).该方法操作简便、准确,可作为四味土木香散及其制剂的质量控制方法.
提齣瞭同時測定四味土木香散中苦參堿和氧化苦參堿含量的方法.採用高效毛細管電泳法.實驗條件為:用緩遲溶液20 mmol/L Tris-40 mmol/L NaH_2PO_4-20%異丙醇(用燐痠調到pH 5.5)作為電泳液,檢測波長為200 nm.被測樣品中苦參堿和氧化苦參堿完全分離且被準確測定.其平均加樣迴收率分彆為99.2%和100.1%,RSD分彆為2.31%和1.57%(n=9).該方法操作簡便、準確,可作為四味土木香散及其製劑的質量控製方法.
제출료동시측정사미토목향산중고삼감화양화고삼감함량적방법.채용고효모세관전영법.실험조건위:용완충용액20 mmol/L Tris-40 mmol/L NaH_2PO_4-20%이병순(용린산조도pH 5.5)작위전영액,검측파장위200 nm.피측양품중고삼감화양화고삼감완전분리차피준학측정.기평균가양회수솔분별위99.2%화100.1%,RSD분별위2.31%화1.57%(n=9).해방법조작간편、준학,가작위사미토목향산급기제제적질량공제방법.
An HPCE method for simultaneous determination of matrine and oxymatrine in siweitumuxiangs powder was established.A high performance capillary electrophoresis method was utilized.The buffer solution used in this method composed of 20 mmol/L of TRIS,40 mmol/L of NaH_2PO_4 and 20% isopropyl alcohol (with the pH value being adjusted to 5.5 by phosphoric acid),and the detection wavelength was 200 nm.Matrine and oxymatrine in the sample were separated completely and determined accurately.The recoveries and the RSD were 99.2%,2.31% for matrine and 100.1%,1.57%(n=9) for oxymatrine,respectively.This method is simple and accurate,which can be used for the quality control on siweitumuxiang powder and its preparations.
