CAJ | 학술논문

采用SSR分子标记技术对来自12个组合的62份稳定彩色棉新品系进行分子变异分析.结果表明,从242对SSR中筛选出的29对多态性引物在供试材料中共扩增出198个标记.其中,多态性标记115个,占总标记数的58.1%.这些引物能较好地揭示彩色棉的变异位点.MGHES-6引物扩增的多态性标记最多,其次为BNL2960、CM43和MGHES-66.29对SSR引物的PIC值变化在0.493～0.938之间,平均0.790.说明彩色棉SSR等位基因丰富度较高.基于SSR数据的聚类分析,可将62份新品系分为两大类,一类是由彩色棉种质资源经系统选育获得的材料,另一类是通过杂交选育获得的材料.
채용SSR분자표기기술대래자12개조합적62빈은정채색면신품계진행분자변이분석.결과표명,종242대SSR중사선출적29대다태성인물재공시재료중공확증출198개표기.기중,다태성표기115개,점총표기수적58.1%.저사인물능교호지게시채색면적변이위점.MGHES-6인물확증적다태성표기최다,기차위BNL2960、CM43화MGHES-66.29대SSR인물적PIC치변화재0.493～0.938지간,평균0.790.설명채색면SSR등위기인봉부도교고.기우SSR수거적취류분석,가장62빈신품계분위량대류,일류시유채색면충질자원경계통선육획득적재료,령일류시통과잡교선육획득적재료.
In the study, SSR molecular marker was used to assess the molecular variation of 62 new lines of colored cottons from 12 combinations. The results showed that 29 of 242 pairs of SSR primers could generate polymorphic markers among the tested colored cotton lines. The total number of markers was 195, and the polymorphic markers were 115, accounted for 58.1%.MGHES-6 primer had the most polymorphie markers, followed by BNL2960, CM43 and MGHES-66. These primers could detect the variable SSR sites of the tested cottons very well. The PIC values for SSR primers varied from 0.493～0.938, averaged 0.790. The higher PIC values showed the abundant SSR alleles from the colored cottons. According to the SSR markers, 62 colored cottons were divided into two groups based on UPGMA method. One group included all the lines bred by pedigree selection, the other included the lines bred from hybridization method. The above results laid a foundation for breeding and germplasm utilization of colored cotton.