CAJ | 학술논문

概述了植物抗真菌病害、细菌病害和病毒病害基因工程育种的主要策略,重点介绍了大豆转基因抗病育种的主要成果.植物抗病害转基因育种策略主要包括基于寄主-病原菌相互识别和信号传导体系的基因工程和基于抗菌蛋白的基因工程;植物抗病毒病害基因工程育种策略还包括病毒外壳蛋白基因介导的抗性、复制酶介导的抗性、运动蛋白介导的抗性、卫星RNA介导的抗性、RNA介导的抗性等.
개술료식물항진균병해、세균병해화병독병해기인공정육충적주요책략,중점개소료대두전기인항병육충적주요성과.식물항병해전기인육충책략주요포괄기우기주-병원균상호식별화신호전도체계적기인공정화기우항균단백적기인공정;식물항병독병해기인공정육충책략환포괄병독외각단백기인개도적항성、복제매개도적항성、운동단백개도적항성、위성RNA개도적항성、RNA개도적항성등.
This paper reviewed strategies in disease resistance breeding by genetic engineering. Then the research progress on disease-resistant breeding of soybean by transgenic technology was summarized. The breeding strategies for disease resistance with genetic engineering can be grouped into two general categories: ( 1) The expression of genes that participate in the host-virus interaction or signal transduction of the hypersensitive response. (2)The expression of gene products that are directly toxic to pathogens or that reduce their growth. These include pathogenesis-related proteins (PR proteins) ,antifungal proteins,antimicrobial peptides,ribosome inactivating proteins( RIP) .and phytoalexins. Moreover,virus-resistant transgenics can be developed by introducing either viral coat protein(CP)or replicase gene encoding sequences. RNA-mediated resistance, known as post-transcriptional gene silencing,is a successful strategy. Other approaches for achieving viral resistance described in the article, include the use of satellite RNA and viral movement protein.