CAJ | 학술논문

背景: 大量研究表明增强脑内源性神经细胞的增殖能力和自我修复将成为治愈缺血缺氧性脑损伤有价值的方法之一.目的:观察氦氖激光对新生大鼠缺血缺氧性脑损伤内源性神经干细胞增殖分化及脑功能重建的影响.方法:7 d龄健康Wistar新生大鼠,建立缺血缺氧性脑损伤模型后第2天开始,激光穴位照射组给予氦氖激光照射.穴位选取顶骨正中的"百会"穴,以及第7颈椎与第1胸椎间、背部正中的"大椎"穴.假手术组和模型组不给予激光照射.于第2疗程结束后,用Y-型迷宫检测各组大鼠的学习记忆能力.随后制备脑海马切片,分别进行巢蛋白和微管关联蛋白2免疫组织化学染色.结果与结论:①激光穴位照射组大鼠的学习和记忆能力明显高于模型组(P < 0.05),但与假手术组相比,无明显差异(P > 0.05).②大鼠内源性神经干细胞的表达:与假手术组比较,模型组、激光治疗组齿状回内巢蛋白免疫阳性细胞均明显增多(P < 0.05),且激光治疗组增多幅度大于模型组(P < 0.05).③神经元特有结构蛋白的表达:激光治疗组大脑皮质微管关联蛋白2表达相当广泛,强阳性染成棕褐色的树突呈条索样、流星样放射状分布,海马各区锥体神经元和齿状回颗粒细胞层神经元排列比较整齐,树突连续阳性染色呈树枝状交叉分布于分子层.假手术组与激光治疗组染色所见无明显差别.模型组微管关联蛋白2表达明显减弱.结果提示激光治疗能够促进缺血缺氧性脑损伤新生大鼠脑内源性神经干细胞增殖,诱导其向神经元方向分化,并达到学习记忆功能的重建.
배경: 대량연구표명증강뇌내원성신경세포적증식능력화자아수복장성위치유결혈결양성뇌손상유개치적방법지일.목적:관찰양내격광대신생대서결혈결양성뇌손상내원성신경간세포증식분화급뇌공능중건적영향.방법:7 d령건강Wistar신생대서,건립결혈결양성뇌손상모형후제2천개시,격광혈위조사조급여양내격광조사.혈위선취정골정중적"백회"혈,이급제7경추여제1흉추간、배부정중적"대추"혈.가수술조화모형조불급여격광조사.우제2료정결속후,용Y-형미궁검측각조대서적학습기억능력.수후제비뇌해마절편,분별진행소단백화미관관련단백2면역조직화학염색.결과여결론:①격광혈위조사조대서적학습화기억능력명현고우모형조(P < 0.05),단여가수술조상비,무명현차이(P > 0.05).②대서내원성신경간세포적표체:여가수술조비교,모형조、격광치료조치상회내소단백면역양성세포균명현증다(P < 0.05),차격광치료조증다폭도대우모형조(P < 0.05).③신경원특유결구단백적표체:격광치료조대뇌피질미관관련단백2표체상당엄범,강양성염성종갈색적수돌정조색양、류성양방사상분포,해마각구추체신경원화치상회과립세포층신경원배렬비교정제,수돌련속양성염색정수지상교차분포우분자층.가수술조여격광치료조염색소견무명현차별.모형조미관관련단백2표체명현감약.결과제시격광치료능구촉진결혈결양성뇌손상신생대서뇌내원성신경간세포증식,유도기향신경원방향분화,병체도학습기억공능적중건.
BACKGROUND: Numerous studies have shown that the increase of reproductive activity and self-repair capacity of brain endogenous neural cells might be a valuable method to treat ischemia-hypoxia brain damage. OBJECTIVE: To study the effects of He-Ne laser irradiation on the proliferation and differentiation of endogenous neural stem cells and brain functional reconstruction in newborn rats with hypoxia-ischemia brain damage.METHODS: Newborn rats aged 7 days were prepared for hypoxia-ischemia brain damage models. From the second day of model induction, rats in the laser treatment group were subjected to He-Ne laser irradiation. Acupuncture point included Baihui (DU20) on the median parietal bone, and Dazhui (GV14) between the C7 and T1 and the median back. After the second course, the learning and memory ability of rats were tested by Y-type maze test. Then brain hippocampal sections were made and underwent immunohistochemistry for nestin and microtubule-associated protein-2.RESULTS AND CONCLUSION: In the laser treatment group, the ability of learning and memory were obviously higher than those in the model group (P < 0.05), however, compared with the sham-operated group, the difference was not obviously (P > 0.05). Compared with the sham-operated group, nestin expression in the dentate gyrus was significantly increased in the model and laser treatment groups (P < 0.05), and the increased range was greater in the laser treatment group compared with the model group (P < 0.05). Microtubule-associated protein-2 expression was widely distributed in the cerebral cortex, and darkly stained brown dendrite presented with radiation-shape. Neurons in the hippocampal pyramid and dentate gyrus granular cell layer arranged regularly. Positively stained dendrite presented branch-shape and distributed in the molecular layer. No significant difference was determined between the sham-operated and laser treatment groups. But the microtubule-associated protein-2 expression was significantly weakened in the model group. He-Ne laser irradiation can promote proliferation of endogenous neural stem cells in neonatal rats with hypoxia-ischemia brain damage, induce its differentiation into neurons, thus, achieves reconstruction of learning and memory functions.