化学研究与应用
化學研究與應用
화학연구여응용
CHEMICAL RESEARCH AND APPLICATION
2010年
11期
1361-1365
,共5页
牛血红蛋白%荧光光谱%圆二色谱%热力学参数
牛血紅蛋白%熒光光譜%圓二色譜%熱力學參數
우혈홍단백%형광광보%원이색보%열역학삼수
hemoglobin%fluorescence spectra%CD spectra%thermodynamic parameters
运用荧光光谱、紫外-可见吸收光谱和圆二色谱法研究了双十二烷基二甲基溴化铵(DDAB)与牛血红蛋白(Hb)的相互作用.从紫外-可见吸收光谱观察到,随着DDAB的浓度增大,Hb在406nm处的特征吸收峰强度下降,且峰位蓝移,说明DDAB导致血红素辅基微观环境变化.由荧光光谱研究可以得出随着DDAB的浓度增大,Hb在340nm处的荧光强度逐渐增强,说明导致色氨酸荧光淬灭的血红素辅基与色氨酸的距离增大.由Scatchard方程计算了不同温度下该反应的表观结合常数、结合位点数及结合热力学参数,热力学参数的变化表明DDAB与Hb之间以疏水作用力为主.圆二色谱的研究进一步表明DDAB使Hb产生轻微的二级结构改变,α-螺旋含量增加.
運用熒光光譜、紫外-可見吸收光譜和圓二色譜法研究瞭雙十二烷基二甲基溴化銨(DDAB)與牛血紅蛋白(Hb)的相互作用.從紫外-可見吸收光譜觀察到,隨著DDAB的濃度增大,Hb在406nm處的特徵吸收峰彊度下降,且峰位藍移,說明DDAB導緻血紅素輔基微觀環境變化.由熒光光譜研究可以得齣隨著DDAB的濃度增大,Hb在340nm處的熒光彊度逐漸增彊,說明導緻色氨痠熒光淬滅的血紅素輔基與色氨痠的距離增大.由Scatchard方程計算瞭不同溫度下該反應的錶觀結閤常數、結閤位點數及結閤熱力學參數,熱力學參數的變化錶明DDAB與Hb之間以疏水作用力為主.圓二色譜的研究進一步錶明DDAB使Hb產生輕微的二級結構改變,α-螺鏇含量增加.
운용형광광보、자외-가견흡수광보화원이색보법연구료쌍십이완기이갑기추화안(DDAB)여우혈홍단백(Hb)적상호작용.종자외-가견흡수광보관찰도,수착DDAB적농도증대,Hb재406nm처적특정흡수봉강도하강,차봉위람이,설명DDAB도치혈홍소보기미관배경변화.유형광광보연구가이득출수착DDAB적농도증대,Hb재340nm처적형광강도축점증강,설명도치색안산형광쉬멸적혈홍소보기여색안산적거리증대.유Scatchard방정계산료불동온도하해반응적표관결합상수、결합위점수급결합열역학삼수,열역학삼수적변화표명DDAB여Hb지간이소수작용력위주.원이색보적연구진일보표명DDAB사Hb산생경미적이급결구개변,α-라선함량증가.
The interaction between hemoglobin (Hb) and didodecyldimethylammonium bromide (DDAB) was investigated by UV-vis spectra, fluorescence spectra and circular dichroism spectra (CD). With the addition of DDAB into the solution of Hb, the Soret absorption decreased gradually, the fluorescence intensity of Hb increased remarkably and α-helical content of Hb increased. According to Scatchard analysis, the binding constants of DDAB to Hb were obtained at different temperature. The parameters of thermodynamics, ΔH, ΔS and ΔG, indicated that the hydrophobic force should be mainly interaction of DDAB with Hb.
