CAJ | 학술논문

目的探讨正常大鼠肝射频消融(radio-frequency ablation,RFA)前后脾组织中单个核细胞表达OX62、OX6及CD86的变化和意义.方法 18只健康SD大鼠均分为RFA 1周组(6只)、RFA 2周组(6只)和对照组(6只),分别在术前、术后1周、术后2周处死动物取出脾组织,采用流式细胞术检测大鼠脾单个核细胞OX62、OX6及CD86表达.同时做病理形态学观察.结果鼠肝组织射频灶病理组织学检查显示凝固性坏死的特点.正常大鼠的脾单个核细胞中(10.36±3.21)%表达大鼠特异性标志OX-62,RFA后1周为(18.03±5.70)%,RFA后2周为(12.63±8.0)%,RFA后1周组与正常大鼠组比较差异有统计学意义.OX-6在正常大鼠脾单个核细胞中的阳性表达率为(76.33 4±7.86)%,RFA后1周为(78.33±7.25)%,RFA后2周为(86.04±7.25)%,RFA后2周组与正常大鼠组比较差异有统计学意义.CD86在正常大鼠脾单个核细胞中的阳性表达率为(63.06±8.77)%,RFA后1周为(55.74±14.49)%,RFA后2周为(63.49±11.81)%.RFA后1周组及RFA后2周组与正常大鼠组比较差异均无统计学意义.结论 RFA促使正常大鼠外周血中树突状细胞前体细胞迁移到脾组织中的数量增多,并进一步分化成熟,对提高机体在免疫应答中的抗原提呈能力起到促进作用.
목적 탐토정상대서간사빈소융(radio-frequency ablation,RFA)전후비조직중단개핵세포표체OX62、OX6급CD86적변화화의의.방법 18지건강SD대서균분위RFA 1주조(6지)、RFA 2주조(6지)화대조조(6지),분별재술전、술후1주、술후2주처사동물취출비조직,채용류식세포술검측대서비단개핵세포OX62、OX6급CD86표체.동시주병리형태학관찰.결과 서간조직사빈조병리조직학검사현시응고성배사적특점.정상대서적비단개핵세포중(10.36±3.21)%표체대서특이성표지OX-62,RFA후1주위(18.03±5.70)%,RFA후2주위(12.63±8.0)%,RFA후1주조여정상대서조비교차이유통계학의의.OX-6재정상대서비단개핵세포중적양성표체솔위(76.33 4±7.86)%,RFA후1주위(78.33±7.25)%,RFA후2주위(86.04±7.25)%,RFA후2주조여정상대서조비교차이유통계학의의.CD86재정상대서비단개핵세포중적양성표체솔위(63.06±8.77)%,RFA후1주위(55.74±14.49)%,RFA후2주위(63.49±11.81)%.RFA후1주조급RFA후2주조여정상대서조비교차이균무통계학의의.결론 RFA촉사정상대서외주혈중수돌상세포전체세포천이도비조직중적수량증다,병진일보분화성숙,대제고궤체재면역응답중적항원제정능력기도촉진작용.
Objective To study the change of spleen Dendritic cells in normal rats treated by radio-frequency ablation(RFA). Methods Eighteen healthy SD rats were separated into group 1 week after RFA with 6 rats,group 2 week after RFA with 6 rats and control group with 6 rats. Spleen tissue were taken out respectively before RFA, 1 week after RFA and 2 weeks after RFA. The number and the phenotype of Dendritic cells in spleen were analyzed with flowcytometry. Results Pathologyical examination after RFA showed the characteristic that coagulation necrosis and cellular degeneration and granulation tissue forming appeared from target center to peripheral of the target. (10. 36±3. 21) % of normal rat mononuclear cells in spleen express OX-62, the ratio became (18. 03±5. 7) % 1 week after RFA and (12. 63±8. 0) % 2 weeks after RFA, the difference between group 1 week after RFA and control group was marked. (76. 33±7. 86) % of normal rat mononuclear cells in spleen express OX-6,the ratio became (78.33±7.25)% 1 week after RFA and (86. 04±7. 25) % 2 weeks after RFA, the difference between group 2 weeks after RFA and control group was marked. (63. 06±8. 77) % of normal rat mononuclear cells in spleen express CD86,the ratio was (55. 74±14. 49)% 1 week after RFA and (63.49±11.81)% 2 weeks after RFA,the difference between groups 1 week or 2 weeks after RFA and control group was not marked. Conclusions RFA can increasing the number of precursor Dendritic cells migrating from peripheral blood to spleen, and those cells may furtherly differentiate or maturate, which may be contributed to improve the ability delivery of body to antigen to a certain extent.