中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2008年
3期
179-183
,共5页
商允菊%戴学栋%荆文%杜会芹%叶红燕%尹苗%张晾%张圣强%李继峰%潘杰
商允菊%戴學棟%荊文%杜會芹%葉紅燕%尹苗%張晾%張聖彊%李繼峰%潘傑
상윤국%대학동%형문%두회근%협홍연%윤묘%장량%장골강%리계봉%반걸
受体,LDL%脂代谢障碍%肝脏%动脉粥样硬化%动物实验
受體,LDL%脂代謝障礙%肝髒%動脈粥樣硬化%動物實驗
수체,LDL%지대사장애%간장%동맥죽양경화%동물실험
Receptors,LDL%Lipid metabolism%Liver%Atherosclerosis%Animal experimentation
目的 探讨脂代谢相关基因在低密度脂蛋白受体(LDLR)基因缺失(LDLR-/-)小鼠肝脏中的表达特征及其与血脂紊乱和动脉粥样硬化早期病变的关系.方法 应用RT-PCR技术分析14、30、60和90天龄LDLR-/-与野生型(WT)小鼠靶基因表达差异,并进行血生化及主动脉形态学检测.结果 与同龄WT小鼠相比,LDLR-/-小鼠肝脏中载脂蛋白AⅣ、脂肪酸转运酶和肉碱棕榈酰转移酶Ⅰ的mRNA水平在14天龄时即显著下调(P<0.05);30天龄时载脂蛋白A Ⅰ显著上调,载脂蛋白F则显著下调(P<0.05);60天龄时肝X受体α显著升高(P<0.05),酰基辅酶A氧化酶1在90天龄时显著下调(P<0.05);载脂蛋白A Ⅴ、载脂蛋白E、过氧化物增殖物激活受体α和血管生成素样蛋白3差异无统计学意义(P>0.05).血清总胆固醇、甘油三酯和低密度脂蛋白C含量从14天龄起均显著高于同龄WT小鼠(均P<0.05),并随年龄增长持续升高.结论 上述脂代谢相关基因在幼龄小鼠即发生表达水平的改变,与血脂紊乱及主动脉病变发生过程呈正相关,说明其可能共同参与幼龄小鼠的脂质代谢紊乱,进而影响动脉内皮细胞功能改变乃至动脉粥样硬化早期病变的发生.
目的 探討脂代謝相關基因在低密度脂蛋白受體(LDLR)基因缺失(LDLR-/-)小鼠肝髒中的錶達特徵及其與血脂紊亂和動脈粥樣硬化早期病變的關繫.方法 應用RT-PCR技術分析14、30、60和90天齡LDLR-/-與野生型(WT)小鼠靶基因錶達差異,併進行血生化及主動脈形態學檢測.結果 與同齡WT小鼠相比,LDLR-/-小鼠肝髒中載脂蛋白AⅣ、脂肪痠轉運酶和肉堿棕櫚酰轉移酶Ⅰ的mRNA水平在14天齡時即顯著下調(P<0.05);30天齡時載脂蛋白A Ⅰ顯著上調,載脂蛋白F則顯著下調(P<0.05);60天齡時肝X受體α顯著升高(P<0.05),酰基輔酶A氧化酶1在90天齡時顯著下調(P<0.05);載脂蛋白A Ⅴ、載脂蛋白E、過氧化物增殖物激活受體α和血管生成素樣蛋白3差異無統計學意義(P>0.05).血清總膽固醇、甘油三酯和低密度脂蛋白C含量從14天齡起均顯著高于同齡WT小鼠(均P<0.05),併隨年齡增長持續升高.結論 上述脂代謝相關基因在幼齡小鼠即髮生錶達水平的改變,與血脂紊亂及主動脈病變髮生過程呈正相關,說明其可能共同參與幼齡小鼠的脂質代謝紊亂,進而影響動脈內皮細胞功能改變迺至動脈粥樣硬化早期病變的髮生.
목적 탐토지대사상관기인재저밀도지단백수체(LDLR)기인결실(LDLR-/-)소서간장중적표체특정급기여혈지문란화동맥죽양경화조기병변적관계.방법 응용RT-PCR기술분석14、30、60화90천령LDLR-/-여야생형(WT)소서파기인표체차이,병진행혈생화급주동맥형태학검측.결과 여동령WT소서상비,LDLR-/-소서간장중재지단백AⅣ、지방산전운매화육감종려선전이매Ⅰ적mRNA수평재14천령시즉현저하조(P<0.05);30천령시재지단백A Ⅰ현저상조,재지단백F칙현저하조(P<0.05);60천령시간X수체α현저승고(P<0.05),선기보매A양화매1재90천령시현저하조(P<0.05);재지단백A Ⅴ、재지단백E、과양화물증식물격활수체α화혈관생성소양단백3차이무통계학의의(P>0.05).혈청총담고순、감유삼지화저밀도지단백C함량종14천령기균현저고우동령WT소서(균P<0.05),병수년령증장지속승고.결론 상술지대사상관기인재유령소서즉발생표체수평적개변,여혈지문란급주동맥병변발생과정정정상관,설명기가능공동삼여유령소서적지질대사문란,진이영향동맥내피세포공능개변내지동맥죽양경화조기병변적발생.
Objective To clarify the differential expression of the genes related to the lipid metabolism in the early stage of atherosclerosis in the young LDLR-/- mice of different ages.Methods A RT-PCR assay was used to analyse the gene expression patterns in the livers of LDLR-/- mice and wild type (WT) mice from 14 to 90 days.The characteristics of early lipid deposition in intima were evaluated using biochemical and pathological techniques.Results In LDLR-/- mice,when compared to WT mice,the mRNA level of the apolipoprotein A Ⅳ(apoA Ⅳ),fatty acid translocase(Fat/CD36)and carnitine palmitoyl transferase Ⅰ(CPT Ⅰ) changed prominently at the age of 14-days(P<0.05).At 30 days,the mRNA level of apolipoprotein A Ⅰ(apoA Ⅰ) was up regulated,but apolipoprotein F(apoF),CD36 and CPT Ⅰ were down regulated(P<0.05).At 60 days,the mRNA levels of apoA Ⅰ,CPT Ⅰ and liver Ⅹ receptor α(LXRα) were up regulated,but apoAⅣ was down regulated(P<0.05).At 90 days,the level of the apoA Ⅰ was higher,but the expression of the apoA Ⅳ,apoF and acyl-coenzymeA oxidase 1 (ACOX1) were down regulated (P<0.05),whereas the expression of apolipoprotein A Ⅴ(apoA Ⅴ),apolipoprotein E (apoE),peroxidase proliferator-activated receptor α(PPARα) and angiopoietin-like protein 3(angptl 3) had no significant changes(P>0.05).The serum levels of TC(P<0.05),TG(P<0.05)and LDLC(P<0.05) in LDLR-/- mice were significantly higher than those in wild type mice with the same age.Conclusions The mRNA levels of the apoA Ⅰ,apoA Ⅳ,apoF,FAT/CD36,CPT Ⅰ,ACOX1 and LXRα of the LDLR-/- mice were significantly changed compared to the WT mice.The genes may be of some relevance to the complicated lipid metabolism network,and have effect in the early stage of atherogenesis.