中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2008年
3期
173-175
,共3页
胰腺炎%糖皮质激素%脑损伤
胰腺炎%糖皮質激素%腦損傷
이선염%당피질격소%뇌손상
Pancreatitis%Glucocorticoids%Brain damage
目的 应用十二指肠置管结扎法制作大鼠急性重症胰腺炎(severe acute pancreatitis,SAP)模型,研究糖皮质激素(glucocorticoids,GC)对急性胰腺炎脑损伤的治疗作用.方法 32只Wistar大鼠随机分为正常对照组(A组)、急性胰腺炎组(B组)、颈总动脉治疗组(C组)和侧脑室治疗组(D组),行十二指肠置管结扎术制成SAP模型,实验动物处死后,测量脑组织含水量,胰腺和脑组织进行HE染色,脑组织样本进行TNF-α、IL-2的免疫组化染色和髓鞘特殊染色,进行胰腺病理损害评分和脑微血管白细胞计数.结果 经糖皮质激素治疗后,脑水肿、白细胞计数及有髓神经脱髓鞘均减轻,差异有显著统计学意义(P＜0.05).结论 糖皮质激素对急性胰腺炎脑损伤有预防和治疗作用.
目的 應用十二指腸置管結扎法製作大鼠急性重癥胰腺炎(severe acute pancreatitis,SAP)模型,研究糖皮質激素(glucocorticoids,GC)對急性胰腺炎腦損傷的治療作用.方法 32隻Wistar大鼠隨機分為正常對照組(A組)、急性胰腺炎組(B組)、頸總動脈治療組(C組)和側腦室治療組(D組),行十二指腸置管結扎術製成SAP模型,實驗動物處死後,測量腦組織含水量,胰腺和腦組織進行HE染色,腦組織樣本進行TNF-α、IL-2的免疫組化染色和髓鞘特殊染色,進行胰腺病理損害評分和腦微血管白細胞計數.結果 經糖皮質激素治療後,腦水腫、白細胞計數及有髓神經脫髓鞘均減輕,差異有顯著統計學意義(P＜0.05).結論 糖皮質激素對急性胰腺炎腦損傷有預防和治療作用.
목적 응용십이지장치관결찰법제작대서급성중증이선염(severe acute pancreatitis,SAP)모형,연구당피질격소(glucocorticoids,GC)대급성이선염뇌손상적치료작용.방법 32지Wistar대서수궤분위정상대조조(A조)、급성이선염조(B조)、경총동맥치료조(C조)화측뇌실치료조(D조),행십이지장치관결찰술제성SAP모형,실험동물처사후,측량뇌조직함수량,이선화뇌조직진행HE염색,뇌조직양본진행TNF-α、IL-2적면역조화염색화수초특수염색,진행이선병리손해평분화뇌미혈관백세포계수.결과 경당피질격소치료후,뇌수종、백세포계수급유수신경탈수초균감경,차이유현저통계학의의(P＜0.05).결론 당피질격소대급성이선염뇌손상유예방화치료작용.
Objective To investigate the therapeutic effects of glucocorticoids(GC)on brain damage in rats with severe acute pancreatitis(SAP).Methods Thirty two rats were randomized into the normal control group(Group A),acute pancreatitis group(Group B),common carotid therapeutic group(Group C)and lateral ventricle therapeutic group(Group D).The rats in group C and D were respectively given methylprednisolone through common carotid artery and lateral ventricle. All rats were killed 24r h later to determine the weight of water content in the brain.The pancreatic and brain tissues were stained with HE.Meanwhile,the brain samples were immunohistochemically stained and the medulla sheath specially stained to determine TNF-α and IL-2.Histopathological scoring of pancreas and counting of leukocytes in the brain were conducted.Results Group A had no obvious pathologic changes.Group C and D had fewer TNF-α positive cells in the brain than Group B.IL-2 was negative in each group.Brain edema,leukocyte number and demylination of myelinated nerves in Group C and D were significantly different from those in Group B(P＜0.05).Conclusion Demylination of myelinated nerves is the characteristic change in SAP.Brain edema,leukocyte infiltration and nerve cell degeneration would happen as well.TNF-α plays an important role in the development of SAP.GC has effects on prevention and therapy of brain damage in rats with SAP.
