CAJ | 학술논문

目的调查EV71所致手足口病患儿及其密切接触儿童的病毒携带状况,探讨肠道病毒传播规律,为科学防控手足口病提供参考.方法现场追踪观察18名聚集性患儿及其50名密切接触儿童并采集标本,利用RD细胞分离肠道病毒,实时荧光RT-PCR方法检测肠道病毒核酸.结果在发病后3～6周内,仍有38.89％(7/18)的确诊患儿粪便中可分离到具有感染活性的EV71;最长排毒时间为39d.密切接触儿童中有22.00％(11/50)在采取“两周闭园”措施后开园第1～3周也可分离到EV71活病毒,其检出距同班首例患儿发病的最长时间为49d.结论 EV71可引起相当数量隐性感染,且在患者和/或隐性感染者粪便中较长时间排出,“两周闭园”措施对阻断EV71的传播作用有限.
목적 조사EV71소치수족구병환인급기밀절접촉인동적병독휴대상황,탐토장도병독전파규률,위과학방공수족구병제공삼고.방법 현장추종관찰18명취집성환인급기50명밀절접촉인동병채집표본,이용RD세포분리장도병독,실시형광RT-PCR방법검측장도병독핵산.결과 재발병후3～6주내,잉유38.89％(7/18)적학진환인분편중가분리도구유감염활성적EV71;최장배독시간위39d.밀절접촉인동중유22.00％(11/50)재채취“량주폐완”조시후개완제1～3주야가분리도EV71활병독,기검출거동반수례환인발병적최장시간위49d.결론 EV71가인기상당수량은성감염,차재환자화/혹은성감염자분편중교장시간배출,“량주폐완”조시대조단EV71적전파작용유한.
Objective To investigate the carriage status among HFMD patients with EV71 and their close contacts,and explore affecting factors for continuing circulation of EV71 ;in order to provide evidence for prevention and control of HFMD.Methods Serial samples of 18 definitely diagnosed HFMD cluster cases and their 50 close contacts,were traced and collected within the interval of 1 to 2 weeks,respectively.RD cells were used for enterovirus isolation and real-time RT-PCR for EV71 specific nucleotide.Results live EV71 have been detected in feces from 38.89％ (7/18) cases within 3rd ～6th weeks after onset,the longest excretion time of live EV71 through stool was 39 days post-onset.22.00％ (11/50) close contacts through 1st ～3rd weeks after the two-week kindergarten suspension,showed also live EV71 in samples,the longest duration of shedding for fecal specimens observed was 49 days since the date of onset for first case in their class.Conclusions EV71 may result in large proportion of asymptomatic infections,and may continuously shed from feces of HFMD patients after recovery for a relatively long period.The intervention effectivehess of “two-week kindergarten suspension” is limited in controlling transmission of EV71.