CAJ | 학술논문

目的观察臭氧氧化预处理对大鼠肾脏缺血再灌注损伤导致的细胞凋亡的影响.方法分为3组进行实验:(1)假手术组:切除大鼠右肾,缝合腹壁；(2)缺血再灌注组:切除大鼠右肾后,夹闭左肾动、静脉45 min,然后开放；(3)臭氧氧化预处理组:手术步骤与缺血再灌注组相同,在术前15d开始经直肠吹入氧气和臭氧的混合气体5～5.5 ml(臭氧浓度为50 mg/L,1 mg·kg-1 ·d-1),应用至术前1d.全自动生化分析仪检测3组大鼠血清尿素氮和肌酐,比色法测定血清脂质过氧化产物丙二醛(MDA)和超氧化物歧化酶(SOD).免疫组织化学法检测大鼠肾细胞胞浆细胞色素C(CytC)的表达,蛋白质印迹法检测CytC的含量.逆转录聚合酶链反应法检测肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6 (IL-6) mRNA的表达.结果缺血再灌注和臭氧氧化预处理组血清尿素氮、肌酐和MDA均高于假手术组,而缺血再灌注组的尿素氮、肌酐和MDA又高于臭氧氧化预处理组.缺血再灌注组血清SOD低于假手术组和臭氧氧化预处理组(P＜0.05).臭氧氧化预处理组的肾组织病理学改变较缺血再灌注组轻.假手术组、缺血再灌注组和臭氧氧化预处理组的CytC表达灰度值分别为101.50±18.02、181.00±20.85和156.71±16.82,两两比较,差异均有统计学意义(P＜0.05).臭氧氧化预处理组肾细胞胞浆和线粒体中CytC含量较缺血再灌注组有所下降(P＜0.05).结论臭氧氧化预处理可以减轻肾脏脂质过氧化反应,减少炎症因子的合成,抑制CytC的释放,减轻肾缺血再灌注损伤.
목적 관찰취양양화예처리대대서신장결혈재관주손상도치적세포조망적영향.방법 분위3조진행실험:(1)가수술조:절제대서우신,봉합복벽；(2)결혈재관주조:절제대서우신후,협폐좌신동、정맥45 min,연후개방；(3)취양양화예처리조:수술보취여결혈재관주조상동,재술전15d개시경직장취입양기화취양적혼합기체5～5.5 ml(취양농도위50 mg/L,1 mg·kg-1 ·d-1),응용지술전1d.전자동생화분석의검측3조대서혈청뇨소담화기항,비색법측정혈청지질과양화산물병이철(MDA)화초양화물기화매(SOD).면역조직화학법검측대서신세포포장세포색소C(CytC)적표체,단백질인적법검측CytC적함량.역전록취합매련반응법검측종류배사인자α(TNF-α)、백세포개소-1β(IL-1β)화백세포개소-6 (IL-6) mRNA적표체.결과 결혈재관주화취양양화예처리조혈청뇨소담、기항화MDA균고우가수술조,이결혈재관주조적뇨소담、기항화MDA우고우취양양화예처리조.결혈재관주조혈청SOD저우가수술조화취양양화예처리조(P＜0.05).취양양화예처리조적신조직병이학개변교결혈재관주조경.가수술조、결혈재관주조화취양양화예처리조적CytC표체회도치분별위101.50±18.02、181.00±20.85화156.71±16.82,량량비교,차이균유통계학의의(P＜0.05).취양양화예처리조신세포포장화선립체중CytC함량교결혈재관주조유소하강(P＜0.05).결론 취양양화예처리가이감경신장지질과양화반응,감소염증인자적합성,억제CytC적석방,감경신결혈재관주손상.
Objective To investigate the protective effect of ozone oxidative preconditioning (OzoneOP) on apoptosis induced by acute renal ischemia/reperfusion (I/R) injury in rats.Methods The right kidneys of rats in control group were excised.A rat I/R model was established in I/R group.In OzoneOP group,the renal OzoneOP was induced by rectal insufflation of 5.0～5.5 ml oxygen plus ozone (ozone 50 mg/L,1 mg· kg- 1 · d- 1,once every day).The parameters (blood urea nitrogen and creatine ) of renal function were determined by auto-biochemical analyzer. The concentrations of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured by using chromometry.Cytochrome c (CytC) was examined by using immunohistochemistry and Western blotting.The mRNA expression levels of TNF-α,IL-1β and IL-6 in the renal tissue were detected by using RT-PCR.Results Compared with the control group,the blood urea nitrogen,creatine,MDA and the release of CytC were increased significantly in I/R group.After OzoneOP,the levels of blood urea nitrogen,creatine,MDA,CytC and the mRNA expression levels of TNF-α,IL-1-β and IL-6were significantly decreased and SOD levels were significantly increased.Conclusion OzoneOP could relieve renal I/R injury of rats by improving antioxidation capability,cutting down cytokines contents and inhibiting the release of CytC from mitochondria.