中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2009年
11期
745-748
,共4页
王志敏%丛雅琴%马立宁%胡晓静
王誌敏%叢雅琴%馬立寧%鬍曉靜
왕지민%총아금%마립저%호효정
骨髓增生异常综合征%富半胱氨酸61%血管内皮生长因子%聚合酶链反应
骨髓增生異常綜閤徵%富半胱氨痠61%血管內皮生長因子%聚閤酶鏈反應
골수증생이상종합정%부반광안산61%혈관내피생장인자%취합매련반응
Myelodysplastic syndromes%Cysteiue-rich61%Vascular endothelial growth factor%Reverse transcriptase-polymerase chain reaction
目的 研究富半胱氨酸61(Cyt61)基因在骨髓增生异常综合征(MDS)不同亚型中的表达情况,探讨Cyr61基因在MDS发生、发展及向急性髓系白血病(AML)演变过程中的意义及其与血管内皮生长因子(VEGF)基因的关系.方法 采用RT-PCR和免疫组化SP法对28例MDS患者、12例AML(其中包括4例由MDS转变而来)患者和10例对照者骨髓单个核细胞(BMMNC)中Cyr61和VEGF mRNA和蛋白质水平进行检测.结果 Cyr61和VEGF mRNA在MDS组和AML组中的表达高于对照组,差异有统计学意义.Cyr61和VEGF mRNA在高危型MDS(RAEB-Ⅰ、RAEB-Ⅱ)(0.3998±0.2467,0.4775±0.1342)和AML患者中的表达(0.4594±0.1737,0.4967±0.2324)明显高于低危型MDS(RA、RARS、RCMD)患者中的表达(0.2213±0.1465,0.2872±0.2341)(P值均＜0.05).高危型MDS患者Cyt61和VEGF mRNA的表达水平和AML患者相比差异无统计学意义.MDS患者Cyr61和VEGF蛋白的表达水平高于对照组,差异有统计学意义.高危型MDS患者Cyr61和VEGF蛋白的表达水平[(38.7±2.9)%,(43.2±2.7)%]明显高于低危型MDS患者[(31.4±3.1)%,(33.5±3.4)%](P＜0.05).MDS患者Cyr61和VEGF的表达呈明显正相关关系(r=0.8762,P＜0.01).结论 Cyr61和VEGF可能在MDS骨髓血管生成及病情发展过程中发挥重要作用.
目的 研究富半胱氨痠61(Cyt61)基因在骨髓增生異常綜閤徵(MDS)不同亞型中的錶達情況,探討Cyr61基因在MDS髮生、髮展及嚮急性髓繫白血病(AML)縯變過程中的意義及其與血管內皮生長因子(VEGF)基因的關繫.方法 採用RT-PCR和免疫組化SP法對28例MDS患者、12例AML(其中包括4例由MDS轉變而來)患者和10例對照者骨髓單箇覈細胞(BMMNC)中Cyr61和VEGF mRNA和蛋白質水平進行檢測.結果 Cyr61和VEGF mRNA在MDS組和AML組中的錶達高于對照組,差異有統計學意義.Cyr61和VEGF mRNA在高危型MDS(RAEB-Ⅰ、RAEB-Ⅱ)(0.3998±0.2467,0.4775±0.1342)和AML患者中的錶達(0.4594±0.1737,0.4967±0.2324)明顯高于低危型MDS(RA、RARS、RCMD)患者中的錶達(0.2213±0.1465,0.2872±0.2341)(P值均＜0.05).高危型MDS患者Cyt61和VEGF mRNA的錶達水平和AML患者相比差異無統計學意義.MDS患者Cyr61和VEGF蛋白的錶達水平高于對照組,差異有統計學意義.高危型MDS患者Cyr61和VEGF蛋白的錶達水平[(38.7±2.9)%,(43.2±2.7)%]明顯高于低危型MDS患者[(31.4±3.1)%,(33.5±3.4)%](P＜0.05).MDS患者Cyr61和VEGF的錶達呈明顯正相關關繫(r=0.8762,P＜0.01).結論 Cyr61和VEGF可能在MDS骨髓血管生成及病情髮展過程中髮揮重要作用.
목적 연구부반광안산61(Cyt61)기인재골수증생이상종합정(MDS)불동아형중적표체정황,탐토Cyr61기인재MDS발생、발전급향급성수계백혈병(AML)연변과정중적의의급기여혈관내피생장인자(VEGF)기인적관계.방법 채용RT-PCR화면역조화SP법대28례MDS환자、12례AML(기중포괄4례유MDS전변이래)환자화10례대조자골수단개핵세포(BMMNC)중Cyr61화VEGF mRNA화단백질수평진행검측.결과 Cyr61화VEGF mRNA재MDS조화AML조중적표체고우대조조,차이유통계학의의.Cyr61화VEGF mRNA재고위형MDS(RAEB-Ⅰ、RAEB-Ⅱ)(0.3998±0.2467,0.4775±0.1342)화AML환자중적표체(0.4594±0.1737,0.4967±0.2324)명현고우저위형MDS(RA、RARS、RCMD)환자중적표체(0.2213±0.1465,0.2872±0.2341)(P치균＜0.05).고위형MDS환자Cyt61화VEGF mRNA적표체수평화AML환자상비차이무통계학의의.MDS환자Cyr61화VEGF단백적표체수평고우대조조,차이유통계학의의.고위형MDS환자Cyr61화VEGF단백적표체수평[(38.7±2.9)%,(43.2±2.7)%]명현고우저위형MDS환자[(31.4±3.1)%,(33.5±3.4)%](P＜0.05).MDS환자Cyr61화VEGF적표체정명현정상관관계(r=0.8762,P＜0.01).결론 Cyr61화VEGF가능재MDS골수혈관생성급병정발전과정중발휘중요작용.
Objective To explore the expression of Cysleine-rieh 61 (Cyr61) gene in the different subtypes of myelodysplastic syndromes (MDS),and the significance of Cyr61 in the genesis progression,and transformation of MDS and the relationship between Cyr61 and vascular endothelial grown factor(VEGF).Methods Reverse transeriptase-polymerase chain reaction (RT-PCR) and immunohistochemical S-P were used to detect mRNA and protein expressions of Cyr61 and VEGF in bone marrow mononuelear cells (BMMNC) from 28 MDS,12 acute myeloid leukemia(AML) patients,and 10 normal volunteers.Results Expressions of Cyr61 and VEGF were higher in MDS and AML patients than in controls(P ＜ 0.05).The expressions of Cyr61 and VEGF were significantly higher in high risk group (0.3998± 0.2647,0.4775 ±0.1342)than that in low risk M DS group(0.2213 ± 0.1465,0.2872±0.2341) (P ＜ 0.05),but no significant difference between high risk MDS and AML patients.Expressions of Cyr61 and VEGF protein were higher in MDS patients than in normal controls(P ＜0.05),and were significantly higher in high risk MDS group [(38.7±2.9)%,(43.2±2.7)%]than in low risk group[(31.4±3.1)%,(33.5±3.4)%](P＜0.05).Expressions of Cyr61 and VEGF were significantly correlated(r =0.8762,P ＜0.01).Conclusion Cyr61 and VEGF may play a role in the angiogenesis and pathogenesis of MDS.
