CAJ | 학술논문

利用石蜡切片技术从胚胎学方面对其进行研究,发现陆地棉双隐性核雄性不育系ms5ms6花药的败育时期是在花粉母细胞时期和小孢子发育期,以小孢子发育异常为主.败育特征为:花粉母细胞时期有少量核仁穿壁现象,还有部分小孢子母细胞出现半月形.小孢子时期出现小孢子粘连和少量的五分体,小孢子都没有刺突长出,最后解体、退化.不育花药和可育花药的绒毡层无明显差异,但不育花药的绒毡层分泌胼胝质的功能不正常.
이용석사절편기술종배태학방면대기진행연구,발현륙지면쌍은성핵웅성불육계ms5ms6화약적패육시기시재화분모세포시기화소포자발육기,이소포자발육이상위주.패육특정위:화분모세포시기유소량핵인천벽현상,환유부분소포자모세포출현반월형.소포자시기출현소포자점련화소량적오분체,소포자도몰유자돌장출,최후해체、퇴화.불육화약화가육화약적융전층무명현차이,단불육화약적융전층분비변지질적공능불정상.
The double recessiveness nuclear male-sterile line ms5ms6 of upland cotton (Gossypium hirsutum L.) has been used in cotton seed production, but there is no report on its abortion stage and character.The result of embryology study showed the abortion stages were the development periods of pollen mother cells and microspore, which was the main abortion stage observed using paraffin slice technique under light microscope.The abortion characters of the double recessiveness nuclear male-sterile line ms5ms6 of upland cotton were: low frequency of nucleolus penetration through nucleus envelop and cell wall and part of pollen mother cells appeared in the shape of lunule; microspore adhesion and few of pentads were found during the periods of microspore; there was no spinescent protuberance on the pollen wall, finally the microspore appeared abnormal and breakdown; there was no difference between fertile anther and sterile anther about the tapetum but the function of tapetum producing corpus callosum is abnormal.