CAJ | 학술논문

目的筛选FXR1P相互作用蛋白,探讨FXR1P生物学功能.方法应用酵母双杂交技术,构建诱饵表达载体pGBKT7/FXR1,转化酵母菌AH109,与转化了人胎脑cDNA文库的酵母菌Y187交配筛选阳性克隆并测序.结果重组表达载体pGBKT7/FXR1构建成功:从人胎脑cDNA文库中筛选出5个与FXR1P结合的蛋白基因,分别与5种已知的编码蛋白基因序列同源:胞苷-磷酸-N-乙酰神经氨酸合成酶(CMAS)、铁蛋白重链多肽1(FTH1)、高尔基体自身抗原golgin a亚类4(GOLGA4)、17-β羟类固醇脱氢酶1(HSD17B1)、绒毛膜生长催乳激素1(CSH1).结论为进一步研究FXR1P的功能及脆性X综合征发病机制提供新的线索.
목적 사선FXR1P상호작용단백,탐토FXR1P생물학공능.방법 응용효모쌍잡교기술,구건유이표체재체pGBKT7/FXR1,전화효모균AH109,여전화료인태뇌cDNA문고적효모균Y187교배사선양성극륭병측서.결과 중조표체재체pGBKT7/FXR1구건성공:종인태뇌cDNA문고중사선출5개여FXR1P결합적단백기인,분별여5충이지적편마단백기인서렬동원:포감-린산-N-을선신경안산합성매(CMAS)、철단백중련다태1(FTH1)、고이기체자신항원golgin a아류4(GOLGA4)、17-β간류고순탈경매1(HSD17B1)、융모막생장최유격소1(CSH1).결론 위진일보연구FXR1P적공능급취성X종합정발병궤제제공신적선색.
Objective To screen the proteins interacting with FXR1P for functional investigation of FXR1P.Methods The yeast strain AH 109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain eDNA library.The positive clones were screened and identified by sequence analysis.Results The recombinant expression vector pGBKT7/FXR1 was constructed successfully.Five proteins binding to FXR1P were screened from human fetal brain eDNA library using the yeast two-hybrid system, including CMAS, FTH l, GOLGA4, HSD17B1 and CSH1.Conclusion These results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.