生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
6期
26-29
,共4页
吕静琳%黄爱玲%郑蓉%李殿殿%吕暾
呂靜琳%黃愛玲%鄭蓉%李殿殿%呂暾
려정림%황애령%정용%리전전%려돈
碱性纤维素酶%16S rDNA%细菌
堿性纖維素酶%16S rDNA%細菌
감성섬유소매%16S rDNA%세균
alkaline-cellulose%16S rDNA%bacterium
目的:筛选1株产纤维素酶的细菌.方法:通过对从腐烂朽木及其附近土壤中得到的样品进行富集培养、分离纯化得到16株纤维素分解菌,经刚果红染色鉴定和液体发酵培养后对其进行了菌种初步鉴定及产酶条件的初步优化.结果:获得1株纤维素酶分泌量较高的细菌LT3.结论:LT3为革兰氏阳性菌,菌体成杆状,经发酵优化培养后,较适产酶条件为甘蔗渣20g/L,pH7.0、30℃培养120h,CMC酶活为71.17U/mL,滤纸酶活为33.37U/mL.通过克隆其16S rDNA序列,对其进行系统进化分析,鉴定为蜡状芽孢杆菌.
目的:篩選1株產纖維素酶的細菌.方法:通過對從腐爛朽木及其附近土壤中得到的樣品進行富集培養、分離純化得到16株纖維素分解菌,經剛果紅染色鑒定和液體髮酵培養後對其進行瞭菌種初步鑒定及產酶條件的初步優化.結果:穫得1株纖維素酶分泌量較高的細菌LT3.結論:LT3為革蘭氏暘性菌,菌體成桿狀,經髮酵優化培養後,較適產酶條件為甘蔗渣20g/L,pH7.0、30℃培養120h,CMC酶活為71.17U/mL,濾紙酶活為33.37U/mL.通過剋隆其16S rDNA序列,對其進行繫統進化分析,鑒定為蠟狀芽孢桿菌.
목적:사선1주산섬유소매적세균.방법:통과대종부란후목급기부근토양중득도적양품진행부집배양、분리순화득도16주섬유소분해균,경강과홍염색감정화액체발효배양후대기진행료균충초보감정급산매조건적초보우화.결과:획득1주섬유소매분비량교고적세균LT3.결론:LT3위혁란씨양성균,균체성간상,경발효우화배양후,교괄산매조건위감자사20g/L,pH7.0、30℃배양120h,CMC매활위71.17U/mL,려지매활위33.37U/mL.통과극륭기16S rDNA서렬,대기진행계통진화분석,감정위사상아포간균.
Objective: To obtain a Cellulose-degrading bacteria. Method: Sixteen strains of different microorganisms with ability to degrade cellulose were obtained by screening the decaying deadwood and soil nearby. In the screening program, accumulation culture, isolation, purification, Congo red dying and liquid fermentation were applied successively. Initial optimization of bateria cultivation conditions for cellulose activity were performed. Result: The most potent isolates (strain LT3) was obtained. Conclusion: LT3 strain was identified as Bacillus cereus which was recognized as gram-positive Bacteria with rod-shaped mycelium. Identification was carried out using morphological and biochemical properties along with 16S rDNA partial sequence analysis. Experiments also indicated that the optimum enzyme-producing conditions were pH 7.0 with 20g/L bagasse at 30 degrees for 120h, the CMCase reached 71.17 U/mL and the FPase was 33.37U/mL.