CAJ | 학술논문

在pH 7.8的缓冲溶液中,铕离子、速尿和三辛基氧膦(TOPO)反应生成稳定的三元配合物,该配合物溶解在Triton X-100胶束中. 以280 nm光波激发,配合物发射出铕离子的特征荧光. 分析了三元配合物的紫外及荧光光谱,对配合物的生成及TOPO和Triton X-100的荧光增敏机理进行了探讨. 采用时间分辨荧光法测定配合物的荧光强度,其荧光强度和速尿的浓度在一定范围内呈线性关系,建立了工作曲线的线性回归方程. 方法的检出限为6.6×10~(-8)mol/L,测定精度RSD为1.3% (4.00×10~(-6)mol/L,n=11). 用此方法测定尿液中痕量速尿,回收率为93.0%～103%.
재pH 7.8적완충용액중,유리자、속뇨화삼신기양련(TOPO)반응생성은정적삼원배합물,해배합물용해재Triton X-100효속중. 이280 nm광파격발,배합물발사출유리자적특정형광. 분석료삼원배합물적자외급형광광보,대배합물적생성급TOPO화Triton X-100적형광증민궤리진행료탐토. 채용시간분변형광법측정배합물적형광강도,기형광강도화속뇨적농도재일정범위내정선성관계,건립료공작곡선적선성회귀방정. 방법적검출한위6.6×10~(-8)mol/L,측정정도RSD위1.3% (4.00×10~(-6)mol/L,n=11). 용차방법측정뇨액중흔량속뇨,회수솔위93.0%～103%.
In Tris-HCl buffer solution of pH 7.8 europium ions, furosemide (FR) and trioctylphosphine oxide (TOPO)formed a stable ternary complex which was dissolved in triton X-100 micelles. The complex emits characteristic fluorescence of europium ion with excitation wavelength at 280 nm. According to the UV and fluorescence spectra of the ternary complex the formation of the complex and mechanism of fluorescence enhancement caused by TOPO and Triton X-100 were discussed. Taking the advantage of long fluorescent lifetime of rare-earth complex the fluorescent intensity of the complex was determined by time-resolved fluorometry. A good linearity between the fluorescence intensity of the complex and the concentration of FR within a certain range was obtained and the linear regression equation was established. The detection limit was 6.6×10~(-8)mol/L and RSD was 1.3% for a 4.00×10~(-6)mol/L FR solution (n=11). The method has been applied to the determination of trace furosemide in urine samples with recoveries between 93.0% and 103%. The new method is simple and rapid with high sensitivity and accuracy.