CAJ | 학술논문

[目的]研究生人工老化对向日葵种子活力及生理特性的影响.[方法]以'Sunm20'、'民乐深紫葵'、'大山葵花4'和'吉葵24'4份向日葵种质为材料,研究了高温高湿人工老化(100%RH,45 ℃)处理不同时间(0、2、4、6、8、10 d)对种子发芽率、发芽势、发芽指数、活力指数、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响.[结果]随着向日葵种子老化时间的延长,发芽率、发芽势、发芽指数、活力指数、POD和SOD活性逐渐下降,MDA含量逐渐增加;不同种质的抗老化能力存在差异,其中'Sunm20'比其他3份种质的抗老化能力强,在相同条件下,其种子活力和保护酶活性最高,MDA含量最低,并且在老化过程中,其种子活力变化及保护酶活性变化最慢.[结论]老化过程中保护酶POD、SOD活性的显著降低可能是导致种子活力下降的主要原因,而少量MDA的逐渐积累促进了种子的老化.
[목적]연구생인공노화대향일규충자활력급생리특성적영향.[방법]이'Sunm20'、'민악심자규'、'대산규화4'화'길규24'4빈향일규충질위재료,연구료고온고습인공노화(100%RH,45 ℃)처리불동시간(0、2、4、6、8、10 d)대충자발아솔、발아세、발아지수、활력지수、과양화물매(POD)、초양화물기화매(SOD)활성급병이철(MDA)함량적영향.[결과]수착향일규충자노화시간적연장,발아솔、발아세、발아지수、활력지수、POD화SOD활성축점하강,MDA함량축점증가;불동충질적항노화능력존재차이,기중'Sunm20'비기타3빈충질적항노화능력강,재상동조건하,기충자활력화보호매활성최고,MDA함량최저,병차재노화과정중,기충자활력변화급보호매활성변화최만.[결론]노화과정중보호매POD、SOD활성적현저강저가능시도치충자활력하강적주요원인,이소량MDA적축점적루촉진료충자적노화.
[Objective]The aim was to study the effects of sunflower artificial aging on seed vigor and physiological characteristics. [Method] The varieties of seed germination capacity, vigor of germination, germination index, vigor index, peroxidase (POD) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content for four sunflower germplasms such as 'SunM20', 'Deep Purple Minle', 'Da San Kui Hua 4' and 'Ji Kui 24' were studied under high temperature and high humidity conditions (100% RH, 45 ℃) for different days (0 d, 2 d, 4 d, 6 d, 8 d, 10 d). [Result] The result showed that the germination capacity, vigor of germination, germination index, vigor index, POD activity, SOD activity declined gradually with the increase of seed aging days, whereas MDA content enhanced by degrees; The diggerences of resistance to artifical aging existed among the four accessions, 'SunM20' was the most resistant one, and exhibited the strongest seed vigor, highest activities of two protective enzyme (POD, SOD) and lowest content of MDA at the uniform condition, moreover, the seed vigor and protective enzyme activities of 'SunM20' changed slowest among the four materials during the aging process. [Conclusion] The distinct reduction of POD, SOD activities maybe the main reasons for the decrease of sunflower seed vigor at the artificial aging, and the gradual accumulation of a few MDA accelerated seeds aging.