中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2012年
3期
245-247
,共3页
黎四平%刘绍基%陆小梅%姚燕红%周艳香
黎四平%劉紹基%陸小梅%姚燕紅%週豔香
려사평%류소기%륙소매%요연홍%주염향
肺炎支原体肺炎%T细胞亚群%免疫球蛋白%补体%儿童
肺炎支原體肺炎%T細胞亞群%免疫毬蛋白%補體%兒童
폐염지원체폐염%T세포아군%면역구단백%보체%인동
Mycoplasma pneumoniae pneumonia%T lymphocyte subsets%Inmunoglobulin%Complement%Children
目的 探讨小儿肺炎支原体肺炎(mycoplasma pneumoniae pneumonia,MPP)不同病期T细胞亚群、免疫球蛋白、补体的变化及其临床意义.方法 应用流式细胞术、免疫散射比浊法检测28例MPP患儿急性期及恢复期外周血T细胞亚群(CD3、CD4、CD8)、免疫球蛋白(IgG、IgA、IgM)、补体(C3、C4)水平,并与25例健康儿童(对照组)进行比较.结果 MPP患儿急性期外周血CD3、CD4、CD8、CD4/CD8分别为(58.71±11.63)％、(32.36±8.06)％、(28.19±6.23)％、1.15±0.41,恢复期分别为(61.29±10.17)％、(34.14±7.22)％、(26.47±6.01)％、1.29±0.37.急性期与恢复期MPP患儿CD4、CD4/CD8比值均低于对照组[(39.53±6.16)％、1.83±0.49],CD8水平高于对照组(1.83±0.49),差异均有统计学意义(P均＜0.01).急性期CD3水平与对照组[(63.03±12.32)％]比较差异有统计学意义(P＜0.01),而恢复期无明显差异(P＞0.05).MPP患儿急性期外周血免疫球蛋白与对照组比较,血清IgG[(14.50±3.86) g/L]、IgM[(1.67±0.56) g/L]与对照组[(7.92±2.62) g/L、(1.06±0.32)g/L]比较明显增高,C3[ (0.83±0.42) g/L]水平低于对照组[(1.37±0.33) g/L],差异均有统计学意义(P＜0.05)；而IgA、C4水平与对照组比较差异无统计学意义(P＞0.05).结论 MPP患儿存在细胞免疫和体液免疫失调.检测T细胞亚群、免疫球蛋白、补体的变化,有利于判断临床治疗效果,为临床应用免疫调节剂提供理论依据.
目的 探討小兒肺炎支原體肺炎(mycoplasma pneumoniae pneumonia,MPP)不同病期T細胞亞群、免疫毬蛋白、補體的變化及其臨床意義.方法 應用流式細胞術、免疫散射比濁法檢測28例MPP患兒急性期及恢複期外週血T細胞亞群(CD3、CD4、CD8)、免疫毬蛋白(IgG、IgA、IgM)、補體(C3、C4)水平,併與25例健康兒童(對照組)進行比較.結果 MPP患兒急性期外週血CD3、CD4、CD8、CD4/CD8分彆為(58.71±11.63)％、(32.36±8.06)％、(28.19±6.23)％、1.15±0.41,恢複期分彆為(61.29±10.17)％、(34.14±7.22)％、(26.47±6.01)％、1.29±0.37.急性期與恢複期MPP患兒CD4、CD4/CD8比值均低于對照組[(39.53±6.16)％、1.83±0.49],CD8水平高于對照組(1.83±0.49),差異均有統計學意義(P均＜0.01).急性期CD3水平與對照組[(63.03±12.32)％]比較差異有統計學意義(P＜0.01),而恢複期無明顯差異(P＞0.05).MPP患兒急性期外週血免疫毬蛋白與對照組比較,血清IgG[(14.50±3.86) g/L]、IgM[(1.67±0.56) g/L]與對照組[(7.92±2.62) g/L、(1.06±0.32)g/L]比較明顯增高,C3[ (0.83±0.42) g/L]水平低于對照組[(1.37±0.33) g/L],差異均有統計學意義(P＜0.05)；而IgA、C4水平與對照組比較差異無統計學意義(P＞0.05).結論 MPP患兒存在細胞免疫和體液免疫失調.檢測T細胞亞群、免疫毬蛋白、補體的變化,有利于判斷臨床治療效果,為臨床應用免疫調節劑提供理論依據.
목적 탐토소인폐염지원체폐염(mycoplasma pneumoniae pneumonia,MPP)불동병기T세포아군、면역구단백、보체적변화급기림상의의.방법 응용류식세포술、면역산사비탁법검측28례MPP환인급성기급회복기외주혈T세포아군(CD3、CD4、CD8)、면역구단백(IgG、IgA、IgM)、보체(C3、C4)수평,병여25례건강인동(대조조)진행비교.결과 MPP환인급성기외주혈CD3、CD4、CD8、CD4/CD8분별위(58.71±11.63)％、(32.36±8.06)％、(28.19±6.23)％、1.15±0.41,회복기분별위(61.29±10.17)％、(34.14±7.22)％、(26.47±6.01)％、1.29±0.37.급성기여회복기MPP환인CD4、CD4/CD8비치균저우대조조[(39.53±6.16)％、1.83±0.49],CD8수평고우대조조(1.83±0.49),차이균유통계학의의(P균＜0.01).급성기CD3수평여대조조[(63.03±12.32)％]비교차이유통계학의의(P＜0.01),이회복기무명현차이(P＞0.05).MPP환인급성기외주혈면역구단백여대조조비교,혈청IgG[(14.50±3.86) g/L]、IgM[(1.67±0.56) g/L]여대조조[(7.92±2.62) g/L、(1.06±0.32)g/L]비교명현증고,C3[ (0.83±0.42) g/L]수평저우대조조[(1.37±0.33) g/L],차이균유통계학의의(P＜0.05)；이IgA、C4수평여대조조비교차이무통계학의의(P＞0.05).결론 MPP환인존재세포면역화체액면역실조.검측T세포아군、면역구단백、보체적변화,유리우판단림상치료효과,위림상응용면역조절제제공이론의거.
Objective To investigate the changes and functions of T lymphocyte subsets,immune globulin and complement in children with mycoplasma pneumoniae pneumonia(MPP) on different disease stages.Methods The levels of T Iymphocyte subsets of CD3,CD4,CD8 and immunoglobulin ( IgG,IgA IgM),and complement ( C3,C4 ) in the peripheral blood were detected on acute and recovery stages in 28 children with MPP by flow cytometry and immune nephelometry.Twenty-five healthy children were recruited as control group.Results Among these subjects of MPP children on acute stage,the levels of CD3,CD4,CD8,and CD4/CD8 in the peripheral blood were (58.71 ± 11.63)％,(32.36 ± 8.06)％,(28.19±6.23 ) ％ and 1.15 ± 0.41 respectively,and on recovery stage,the levels of CD3,CD4,CD8,and CD4/CD8 were (61.29 ±10.17)％,(34.14 ±7.22)％,(26.47 ±6.01)％,and 1.29 ±0.37 respectively.Both on acute stage and on recovery stage of MPP children,the levels of CD4,CD4/CD8 were significantly lower than those in control group [ (39.53 ± 6.16 ) ％,1.83 ± 0.49 ],and CD8 was significantly higher compared to the control group( 1.83 ± 0.49 ),P＜0.01.CD3 were lower than that in control group [ (63.03 ± 12.32) ％ ] on acute stage (P＜0.01 ),and no significant difference on recovery stage (P＞0.05).During the acute stage of MPP,IgG [ ( 14.50 ±3.86) g/L] and IgM [ ( 1.67 ±0.56) g/L] were obviously higher than those in control group [ (7.92 ± 2.62 ) g/L,( 1.06 ± 0.32 ) g/L,P＜0.01 ],and C3 [ ( 0.83 ± 0.42 ) g/L ] were obviously lower compared to the control group [ ( 1.37 ± 0.33 ) g/L,P＜0.05].There were no significant differences of IgA and C4 between MPP and control groups ( P＞0.05 ).Conclusion Children with MPP had celhilar immune and humoral immune disorders.Through the detection of T lymphocyte subsets,immunoglobulin and complement,it will be helpful to judge the effectiveness of clinical treatment,which provides a theoretical basis for the clinical application of immune regulators.
