CAJ | 학술논문

目的探讨补肾排毒合剂对肾间质纤维化的防治作用及其可能的作用机制.方法将60只清洁级昆明小鼠随机分为正常组、单侧输尿管结扎组(UUO组)、补肾排毒合剂组和洛汀新组,采用单侧输尿管结扎术构建肾间质纤维化模型,术后分别于7、14、21、28 d处死小鼠,取术侧肾组织行HE染色,免疫组化法测定肾组织中整合素β1的表达.结果整合素β1在正常肾组织中有微弱表达,UUO术后整合素β1表达高于正常组,补肾排毒合剂组、洛汀新组各时间点较UUO组降低,补肾排毒合剂组与洛汀新组比较差异无统计学意义.结论补肾排毒合剂可通过调节整合素β1的表达,缓解肾间质纤维化.
목적 탐토보신배독합제대신간질섬유화적방치작용급기가능적작용궤제.방법 장60지청길급곤명소서수궤분위정상조、단측수뇨관결찰조(UUO조)、보신배독합제조화락정신조,채용단측수뇨관결찰술구건신간질섬유화모형,술후분별우7、14、21、28 d처사소서,취술측신조직행HE염색,면역조화법측정신조직중정합소β1적표체.결과 정합소β1재정상신조직중유미약표체,UUO술후정합소β1표체고우정상조,보신배독합제조、락정신조각시간점교UUO조강저,보신배독합제조여락정신조비교차이무통계학의의.결론 보신배독합제가통과조절정합소β1적표체,완해신간질섬유화.
Objective To study the therapeutic effects and its possible mechanism ofBushen Paidu Mixture on renal interstitial fibrosis. Methods A total of 60 mice were randomly divided into a normal group,UUO group, Bushen Paidu Mixture group and Lotensin group. Mice models were induced by unilateral ureteral occlusion (UUO). Execute the mice at 7, 14, 21, and 28 days after UUO. The involved kidney were separated and had HE staining. Determine integrinβ1 in kidney tissue through immunohistochemical methods. Results Integrinβ1 tissues had a weak expression in normal kidney. Integrinβ1 expressed higher in UUO group than normal group. The expression of integrinβ1 in Bushen Paidu Mixture group and Lotensin group was lower than the UUO group at every timing point. There is no significant difference between the Bushen Mixture group and Lotensin group in terms of integrinβ1 expression. Conclusion Bushen Paidu Mixture can slow down the process of renal interstitial fibrosis through regulating integrinβ1 expression.