中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2011年
7期
491-493
,共3页
白癜风%黑素细胞%透明质酸
白癜風%黑素細胞%透明質痠
백전풍%흑소세포%투명질산
Vitiligo%Melanocyte%Hyaluronic acid
目的 观察玻璃酸钠对黑素细胞增殖及酪氨酸酶活性的影响.方法 噻唑蓝法和酪氨酸酶活性测定法分别检测不同浓度玻璃酸钠对正常人黑素细胞生长和黑素合成能力的影响.结果 玻璃酸钠质量浓度为0,0.008、0.016、0.313、0.625、1.250、2.500、5.000、7.500、10.000 g/L时,黑素细胞增殖活性(A490)分别为0.14±0.02、0.37±0.08、0.45±0.11、0.49±0.07、0.55±0.12、0.52±0.11、0.49±0.07、0.39±0.05、0.19±0.03、0.01±0.01,玻璃酸钠质量浓度在0.08～5 g/L范围内时,对黑素细胞的增殖有明显促进作用,达10 g/L时会抑制黑素细胞的增长.玻璃酸钠质量浓度在0.2～5 g/L时可明显促进酪氨酸酶活性,到10 g,L时酪氨酸酶活性反而下降.在接种黑素细胞同时加入玻璃酸钠的黑素细胞增殖作用明显快于接种后8 h再加入玻璃酸钠组.结论 玻璃酸钠在浓度0.2～5 g/L时既能促进黑素细胞的增殖,又能明显提高黑素细胞的酪氨酸酶活性.
目的 觀察玻璃痠鈉對黑素細胞增殖及酪氨痠酶活性的影響.方法 噻唑藍法和酪氨痠酶活性測定法分彆檢測不同濃度玻璃痠鈉對正常人黑素細胞生長和黑素閤成能力的影響.結果 玻璃痠鈉質量濃度為0,0.008、0.016、0.313、0.625、1.250、2.500、5.000、7.500、10.000 g/L時,黑素細胞增殖活性(A490)分彆為0.14±0.02、0.37±0.08、0.45±0.11、0.49±0.07、0.55±0.12、0.52±0.11、0.49±0.07、0.39±0.05、0.19±0.03、0.01±0.01,玻璃痠鈉質量濃度在0.08～5 g/L範圍內時,對黑素細胞的增殖有明顯促進作用,達10 g/L時會抑製黑素細胞的增長.玻璃痠鈉質量濃度在0.2～5 g/L時可明顯促進酪氨痠酶活性,到10 g,L時酪氨痠酶活性反而下降.在接種黑素細胞同時加入玻璃痠鈉的黑素細胞增殖作用明顯快于接種後8 h再加入玻璃痠鈉組.結論 玻璃痠鈉在濃度0.2～5 g/L時既能促進黑素細胞的增殖,又能明顯提高黑素細胞的酪氨痠酶活性.
목적 관찰파리산납대흑소세포증식급락안산매활성적영향.방법 새서람법화락안산매활성측정법분별검측불동농도파리산납대정상인흑소세포생장화흑소합성능력적영향.결과 파리산납질량농도위0,0.008、0.016、0.313、0.625、1.250、2.500、5.000、7.500、10.000 g/L시,흑소세포증식활성(A490)분별위0.14±0.02、0.37±0.08、0.45±0.11、0.49±0.07、0.55±0.12、0.52±0.11、0.49±0.07、0.39±0.05、0.19±0.03、0.01±0.01,파리산납질량농도재0.08～5 g/L범위내시,대흑소세포적증식유명현촉진작용,체10 g/L시회억제흑소세포적증장.파리산납질량농도재0.2～5 g/L시가명현촉진락안산매활성,도10 g,L시락안산매활성반이하강.재접충흑소세포동시가입파리산납적흑소세포증식작용명현쾌우접충후8 h재가입파리산납조.결론 파리산납재농도0.2～5 g/L시기능촉진흑소세포적증식,우능명현제고흑소세포적락안산매활성.
Objective To observe the effect of hyaluronic acid on the proliferation of and tyrosinase activity in melanocytes.Methods Normal primary human melanocytes were isolated from infant foreskin tissue and cultured.Different concentrations(0 to 10 g/L)of hyaluronic acid wero added to the culture medium immediately or 8 hours after the inoculation of melanocytes.MTT assay was performed to detect the proliferation of melanocytes,and tyrosinase activity was determined to evaluate the effect of hyaluronic acid on the melanin synthesis by melanocytes.Results The proliferation level (absorbance at 490 am,A490)of melanocytes was 0.14±0.02,0.37±0.08,0.45±0.11,0.49±0.07,0.55±0.12,0.52±0.11,0.49±0.07,0.39±0.05,0.19±0.03 and 0.01 4-0.01 when treated with hyaluronic acid of 0,0.008,0.016,0.313,0.625,1.250,2.500,5.000,7.500 and 10.000 g/L,respectively.The hyaluronic acid of 0.08 to 5 g/L markedly accelerated the proliferation of melanocytes,while that of 10 g/L inhibited their proliferation.The tyrosinase activity in melanocytes was promoted by hyaluronie acid of 0.2 to 5 g/L,but suppressed by that of 10 g/L.The proliferation of melanocytes treated with hyaluronic acid immediately after the inoculation was more rapid than that treated with hyaluronic acid 8 hours after the inoculation.Conclusion The hyaluronic acid of 0.2 to 5 g/L can enhance the proliferation of and tyrosinase activity in melanocytes.
