CAJ | 학술논문

目的建立兔脑血管痉挛(CVS)基底动脉和正常兔基底动脉双向凝胶电泳图谱,鉴定差异表达蛋白,从中发现有意义的CVS相关标志物.方法采用双向凝胶电泳(2-DE)分离痉挛基底动脉和正常基底动脉总蛋白,银染显色,通过imagemaster5.0软件分析,从中选取差异表达蛋白质点,应用基质辅助激光解析飞行时间串联质谱(MALDI-TOF/TOF)鉴定差异表达的蛋白质.结果获得重复性和分辨率较好的兔基底动脉双向凝胶电泳图谱;发现49个差异表达点,其中35个点得到鉴定,在CVS中高表达的有24个,其余11个在CVS中呈低表达.结论建立了痉挛基底动脉和正常基底动脉双向凝胶电泳图谱,并应用质谱技术鉴定了35个差异表达点,这些差异表达蛋白质可能与CVS的发生相关.
목적 건립토뇌혈관경련(CVS)기저동맥화정상토기저동맥쌍향응효전영도보,감정차이표체단백,종중발현유의의적CVS상관표지물.방법 채용쌍향응효전영(2-DE)분리경련기저동맥화정상기저동맥총단백,은염현색,통과imagemaster5.0연건분석,종중선취차이표체단백질점,응용기질보조격광해석비행시간천련질보(MALDI-TOF/TOF)감정차이표체적단백질.결과 획득중복성화분변솔교호적토기저동맥쌍향응효전영도보;발현49개차이표체점,기중35개점득도감정,재CVS중고표체적유24개,기여11개재CVS중정저표체.결론 건립료경련기저동맥화정상기저동맥쌍향응효전영도보,병응용질보기술감정료35개차이표체점,저사차이표체단백질가능여CVS적발생상관.
Objective To establish two-dimensional electrophoresis (2-DE) profiles of basilar arteries and paired normal basilar arteries of cerebral vasospasm in rabbit to identify differential expression proteins in order to find out candidates as cerebral vasospasm-related proteins. Methods 2-DE was applied to separate the total proteins of cerebral vasospasm basilar arteries and paired normal basilar arteries.After staining and analyzing by imagemaster 5.0 software. The differential proteins were identified by MALDI-TOF/TOF. Results 2-DE profiles with high resolution and reproducibility was obtained. Forty-nine protein spots were found as differentially-expressed proteins, in which 35 protein spots were successfully identified. Among the identified proteins, there were 24 over-expressed and 11 under-expressed proteins in spasm basilar arteries as compared with normal basilar arteries. Conclusions Established 2-DE patterns of rabbit spasm basilar arteries and paired normal basilar arteries and 35 differentially expressed proteins spots are identified, and which may participate in cerebral vasospasm.