CAJ | 학술논문

山杏成熟种子在改良MS培养基上培养获得下胚轴,将其切段后培养在改良MS培养基+TDZ 2.0 mg/L+NAA 0.5 mg/L的再生培养基上,经15 d的暗期培养后,其再生频率可达到37%以上.试验表明分裂素TDZ促进不定芽再生的效果优于6-BA,生长素NAA效果优于2,4-D,2-3周龄的下胚轴再生效果较好,但下胚轴的取材部位对再生无显著影响.
산행성숙충자재개량MS배양기상배양획득하배축,장기절단후배양재개량MS배양기+TDZ 2.0 mg/L+NAA 0.5 mg/L적재생배양기상,경15 d적암기배양후,기재생빈솔가체도37%이상.시험표명분렬소TDZ촉진불정아재생적효과우우6-BA,생장소NAA효과우우2,4-D,2-3주령적하배축재생효과교호,단하배축적취재부위대재생무현저영향.
Hypocotyls rasied from mature seeds of Prunus armeniaca L var. ansu cultured on modified MS medium were cutinto segments and cultured on the regeneration medium, which consisted of modified MS + TDZ 2.0 mg/L+NAA 0.5 mg/L,in dark for 15 d and then exposed to light. The rate of shoot regeneration was higher than 37%. The result showed that TDZwas better than 6-benzylamino-purine (6-BA), and NAA was better than 2,4-D in adventitious shoot regeneration. 2 to 3week-aged hypocotyls produced better result, but the position effect of explants was not significant for shoot regeneration andcallus induction.