CAJ | 학술논문

研究生物可降解材料聚乳酸乙醇酸共聚物(PLGA 90/10)的体外降解特性,通过测量材料在体外降解过程中的重量损失并在扫描电镜下观察形态学变化,评估体外降解特性,该实验是在37℃ pH为7.4的磷酸盐缓冲液中进行的.为研究材料的细胞毒性,将小牛血管平滑肌细胞种植在PLGA膜片上,在显微镜下观察细胞生长情况,并通过MTT法测定细胞活性.研究发现结晶型的PLGA90/10显示出表面降解的特性,高分子量的PLGA90/10在开始的4个月内降解缓慢,120天时重量仅损失10%,120天后迅速降解.MTT 法检测细胞增殖指数,与对照组差异无显著意义.这为局部药物治疗工具提供了基础研究.
연구생물가강해재료취유산을순산공취물(PLGA 90/10)적체외강해특성,통과측량재료재체외강해과정중적중량손실병재소묘전경하관찰형태학변화,평고체외강해특성,해실험시재37℃ pH위7.4적린산염완충액중진행적.위연구재료적세포독성,장소우혈관평활기세포충식재PLGA막편상,재현미경하관찰세포생장정황,병통과MTT법측정세포활성.연구발현결정형적PLGA90/10현시출표면강해적특성,고분자량적PLGA90/10재개시적4개월내강해완만,120천시중량부손실10%,120천후신속강해.MTT 법검측세포증식지수,여대조조차이무현저의의.저위국부약물치료공구제공료기출연구.
We assessed the in vitro degradation of poly(dl-lactic-co-glycolic acid) (PLGA)90L/10G, a biodegradable material. In vitro degradation characteristics were evaluated by measuring decrease in the mass loss of PLGA films and conducted at 37℃ in pH 7.4 phosphate buffered saline. The morphological changes of films surface during degradation were observed on a scanning electron microscopy.The viability of cells was determined by MTT method. PLGA90/10 was hydrophobic polymer and it showed surface erosion characteristics. High-molecular-weight PLGA degraded slowly in the 4 months of beginning; there was 10% mass loss in the 120th day and rapid mass loss after the 120th day. There was no difference on the cell viability between control group and PLGA group. Our study provides a basis for the development of a useful vehicle for locally administered drugs.