CAJ | 학술논문

目的探讨生存素(Survivin)反义寡核苷酸(ASODN)诱导人胃癌细胞SGC7901凋亡的作用.方法设计合成特异性靶向Survivin的ASODN,将胃癌细胞株分为空白对照组(Sham组)、脂质体转染对照组(Lip组)、正义链转染对照组(Lip-SODN组)和ASODN转染组(Lip-ASODN组).作用48 h后,Western blot法检测各组Survivin表达情况,流式细胞仪检测各组细胞凋亡率,免疫组化SP法检测细胞中PCNA表达情况.结果脂质体介导Survivin ASODN转染后的胃癌细胞Survivin蛋白表达明显下降;ASODN转染组细胞凋亡率明显高于各对照组(P均＜0.05),各对照组间无统计学差异(P＞0.05).ASODN转染后胃癌细胞中PCNA表达水平明显降低.结论 Survivin ASODN转染胃癌细胞能下调Survivin蛋白表达,诱导胃癌细胞凋亡,抑制细胞增殖,具有明显的抗癌作用.
목적 탐토생존소(Survivin)반의과핵감산(ASODN)유도인위암세포SGC7901조망적작용.방법 설계합성특이성파향Survivin적ASODN,장위암세포주분위공백대조조(Sham조)、지질체전염대조조(Lip조)、정의련전염대조조(Lip-SODN조)화ASODN전염조(Lip-ASODN조).작용48 h후,Western blot법검측각조Survivin표체정황,류식세포의검측각조세포조망솔,면역조화SP법검측세포중PCNA표체정황.결과 지질체개도Survivin ASODN전염후적위암세포Survivin단백표체명현하강;ASODN전염조세포조망솔명현고우각대조조(P균＜0.05),각대조조간무통계학차이(P＞0.05).ASODN전염후위암세포중PCNA표체수평명현강저.결론 Survivin ASODN전염위암세포능하조Survivin단백표체,유도위암세포조망,억제세포증식,구유명현적항암작용.
Objective To investigate the apoptosis effects of Survivin antisense oligonucleotide (ASODN)on human gastric carcinoma cell SGC7901. Methods ASODN targeting Surviving was designed and constructed. Cultured cells were divided into control group, lipofectin group, oligonucleotide(ODN) group and ASODN group. After transfection for 48 h, Survivin protein expression was detected by Western blot; apoptotic rate (AR) was examined by flow cytometry; the expression of PCNA was detected in gastric cancer cells by immunohistochemical staining. Results The expression of Survivin in SGC7901 cells decreased after transfected with Survivin ASODN. The AR of ASODN group was significantly higher than that of other groups; Immunohistochemical test indicated that transfected ASODN downregulated the expression levels of PCNA. Conclusions Expression of Survivin may decrease in gastric cancer cells after ASODN transfection. ASODN targeting Survivin can induce cell apoptosis, inhibit cell proliferation. Survivin ASODN is a prospective anti-cancer drug