CAJ | 학술논문

目的探讨白藜芦醇(resveratrol,Res)对小鼠3T3-L1细胞增殖、分化的影响及其机制.方法培养3T3-L1前脂肪细胞,添加不同剂量的白藜芦醇,用WST-1法检测细胞的增殖;用油红O染色后以染色比色法分析脂肪细胞的分化程度;采用Real time PCR技术检测各组脂联素和瘦素的mRNA表达;采用Western blot技术检测相关位点,包括沉默信息调节因子1(silent information regulator 1,Sirt1)、过氧化物酶增殖物激活受体γ(peroxisome proliferator activated receptor γ,PPARγ)、CCAAT/增强子结合蛋白α(CCTTA enhancer binding protein α,C/EBPα)的蛋白质表达.结果 Res明显抑制3T3-L1前脂肪细胞增殖,且呈一定的时间-剂量依赖关系;与对照组比较,Res组脂联素和瘦素的mRNA表达水平下降(P<0.01),Res抑制3T3-L1前脂肪细胞向成熟脂肪细胞分化;添加Res导致脂肪细胞中Sirt1蛋白表达水平上升,PPARγ和C/EBPα蛋白表达水平下降.结论白藜芦醇能抑制3T3-L1前脂肪细胞增殖和分化,其机制可能是通过增加Sirt1表达,抑制脂肪细胞分化相关蛋白PPARγ、C/EBPα的表达.
목적 탐토백려호순(resveratrol,Res)대소서3T3-L1세포증식、분화적영향급기궤제.방법 배양3T3-L1전지방세포,첨가불동제량적백려호순,용WST-1법검측세포적증식;용유홍O염색후이염색비색법분석지방세포적분화정도;채용Real time PCR기술검측각조지련소화수소적mRNA표체;채용Western blot기술검측상관위점,포괄침묵신식조절인자1(silent information regulator 1,Sirt1)、과양화물매증식물격활수체γ(peroxisome proliferator activated receptor γ,PPARγ)、CCAAT/증강자결합단백α(CCTTA enhancer binding protein α,C/EBPα)적단백질표체.결과 Res명현억제3T3-L1전지방세포증식,차정일정적시간-제량의뢰관계;여대조조비교,Res조지련소화수소적mRNA표체수평하강(P<0.01),Res억제3T3-L1전지방세포향성숙지방세포분화;첨가Res도치지방세포중Sirt1단백표체수평상승,PPARγ화C/EBPα단백표체수평하강.결론 백려호순능억제3T3-L1전지방세포증식화분화,기궤제가능시통과증가Sirt1표체,억제지방세포분화상관단백PPARγ、C/EBPα적표체.
Aim To explore the effect and mechanism of resveratrol (Res) on proliferation and differentiation of murine 3T3-L1 preadipocytes.Methods 3T3-L1 preadipocytes were cultured and treated with resveratrol in different dosages.Cell proliferation was analyzed by WST-1 method. Oil red O staining method and spectrophotography were applied to analyze the degree of differentiation. Real-time PCR was applied to detect the mRNA expression of adiponectin and leptin. Western blot was applied to detect the expression of silent information regulator 1 (Sirt1),peroxisome proliferator activated receptor γ (PPARγ) and CCTTA enhancer binding proteinα (C/EBPα).Results Res inhibited proliferation of murine 3T3-L1 preadipocytes in a time-dose dependent manner.The expression levels of adiponectin and leptin mRNA were decreased, and Res also inhibited 3T3-L1 preadipocytes to differentiate into mature adipocytes. Res increased the expression levels of Sirt1 and decreased the expression levels of PPARγ and C/EBPα.Conclusions Resveratrol can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes.The underlying mechanisms may include enhancing expression of Sirt1 and inhibiting expression of PPARγ,C/EBPα which are related to cell differentiation.