CAJ | 학술논문

为建立分离纯化鸟鳢体表粘液抗茵肽的方法并研究其抗菌活性,本研究通过嗜水气单胞菌对鸟鳢进行刺激,采用甲醇提取、Sephadex G-50凝胶层析和反向高效液相色谱从其体表粘液中提取抗菌肽,经质谱分析测定分子量;并对抗茵肽的抑茵谱、最小抑茵浓度(~ⅡC)、热稳定性、溶血活性进行了初步研究.结果表明:分离纯化的乌鳢体表粘液抗菌肽分子量约为3 024.15 ku,并具有广谱的抗菌活性,热稳定性好,没有溶血活性.
위건립분리순화조례체표점액항인태적방법병연구기항균활성,본연구통과기수기단포균대조례진행자격,채용갑순제취、Sephadex G-50응효층석화반향고효액상색보종기체표점액중제취항균태,경질보분석측정분자량;병대항인태적억인보、최소억인농도(~ⅡC)、열은정성、용혈활성진행료초보연구.결과표명:분리순화적오례체표점액항균태분자량약위3 024.15 ku,병구유엄보적항균활성,열은정성호,몰유용혈활성.
Antibacterial peptide was extracted from mucus surface of Channa argu by methanol exwaetion,gel chromatography and reversed phase high performance liquid chromatography.The molecular weight of the peptide was determined by the time-of-flight mass spectrometry and its antibacterial spectrum,minimal inhibitory concentration (MIC),thermal stability and hemolytic activity was also examined.The results showed that the antibacterial peptide was about 3 ku,and had a broad-spectrum antimicrobial activity andthermal stability,but no hemolytic activity.