CAJ | 학술논문

目的观察冻存对骨髓基质细胞(bone marrow stromal cells,BMSC)体内形成牙周支持组织能力的影响.方法在5只Beagle犬的26个实验牙位制备牙周缺损.区组随机法分成3组,空白组(8颗)植入载体胶原膜,未冻存组(9颗)和冻存组(9颗)接种未冻存或冻存细胞与胶原膜复合物,8周后观察牙周组织再生情况.结果冻存组和未冻存组牙槽骨再生百分比分别为(83.7±10.6)%、(85.0±6.8)%,两组新生牙骨质和牙周膜百分比均为100%,差异无统计学意义(P>0.05);空白组牙槽骨再生百分比为(24.7±9.2)%,牙骨质再生百分比为(21.0±4.8)%,牙周膜再生百分比为(24.8±5.4)%,均较冻存组和未冻存组显著减少,差异有统计学意义(P<0.05).结论冻存对BMSC体内分化形成牙周组织的能力无显著影响.
목적 관찰동존대골수기질세포(bone marrow stromal cells,BMSC)체내형성아주지지조직능력적영향.방법 재5지Beagle견적26개실험아위제비아주결손.구조수궤법분성3조,공백조(8과)식입재체효원막,미동존조(9과)화동존조(9과)접충미동존혹동존세포여효원막복합물,8주후관찰아주조직재생정황.결과 동존조화미동존조아조골재생백분비분별위(83.7±10.6)%、(85.0±6.8)%,량조신생아골질화아주막백분비균위100%,차이무통계학의의(P>0.05);공백조아조골재생백분비위(24.7±9.2)%,아골질재생백분비위(21.0±4.8)%,아주막재생백분비위(24.8±5.4)%,균교동존조화미동존조현저감소,차이유통계학의의(P<0.05).결론 동존대BMSC체내분화형성아주조직적능력무현저영향.
Objective To investigate the influence of cryopreservation on bone marrow stromal cells'(BMSC)capability of enhancing periodontal regeneration.Methods Twenty-six artificial periodontal defects were established in 5 Beagle dogs and divided into 3 groups at random.Only collagen membrane,the complex of cryopreserved and uncryopreserved BMSC and collagen scaffold were transplanted in the blank control group,cropreserved and uncryopreserved groups respectively.The periodontal regeneration was observed 8 weeks after transplantation.Results The percentage of periodontal regeneration in cryopreserved and uncryopreserved groups was significantly greater than that of the blank control group(P<0.05),but no statistical difierence was found between cryopreserved and uncryopreserved groups.Conclusions Cryopreservation had no significant negative effects on BMSC capability of enhancing periodontal regeneration.