中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2008年
7期
504-508
,共5页
鼻炎%变应性%季节性%模型%动物%半乳糖凝集素类
鼻炎%變應性%季節性%模型%動物%半乳糖凝集素類
비염%변응성%계절성%모형%동물%반유당응집소류
Rhinitis,allergic,seasonal%Models,animal%Galectins
目的 探讨小鼠鼻黏膜内半乳糖凝集素3(galectin-3)和半乳糖凝集素9(galectin-9)的表达水平及其与变应性鼻炎的关系.方法 20只BALB/c小鼠随机分为变应性鼻炎组(AR组)和对照组,每组10只.AR组分别于第1天和第14天腹腔注射含卵清蛋白10μg及氢氧化铝2 mg的生理盐水悬液0.1ml,第21天起鼻腔给予1%卵清蛋白局部激发,持续7 d;对照组用生理盐水代替卵清蛋白.通过免疫组织化学染色和逆转录-聚合酶链反应(RT-PCR)检测鼻黏膜中galectin-3和galectin-9 的表达,同时采用酶联免疫吸附测定法(ELISA)检测外周血中自细胞介素4(IL-4)、白细胞介素5(IL-5)及γ干扰素(IFN-γ)的表达.结果 AR组和对照组均有galectin-3和galectin-9蛋白及mRNA的表达,AR组表达强度高于对照组,差异具有统计学意义(t值分别为11.18、9.261、7.667和6.971,P值均＜0.05).与对照组相比,AR组小鼠外周血中IL-4和IL-5含量均有不同程度的增高(t值分别为7.202和8.121,P值均＜0.05),而IFN-γ含量则下降(t=-11.94,P＜0.05).galectin 3和galectin 9 mRNA的表达强度与外周血中IL-4、IL-5的含量呈正相关(r值分别为0.667、0.847、0.720及0.736,P值均＜0.05),与IFN-γ含量呈负相关(r值分别为-0.528和-0.817,P值均＜0.05).结论 galectin 3和galectin 9可能在变应性鼻炎的发病过程中发挥一定作用.
目的 探討小鼠鼻黏膜內半乳糖凝集素3(galectin-3)和半乳糖凝集素9(galectin-9)的錶達水平及其與變應性鼻炎的關繫.方法 20隻BALB/c小鼠隨機分為變應性鼻炎組(AR組)和對照組,每組10隻.AR組分彆于第1天和第14天腹腔註射含卵清蛋白10μg及氫氧化鋁2 mg的生理鹽水懸液0.1ml,第21天起鼻腔給予1%卵清蛋白跼部激髮,持續7 d;對照組用生理鹽水代替卵清蛋白.通過免疫組織化學染色和逆轉錄-聚閤酶鏈反應(RT-PCR)檢測鼻黏膜中galectin-3和galectin-9 的錶達,同時採用酶聯免疫吸附測定法(ELISA)檢測外週血中自細胞介素4(IL-4)、白細胞介素5(IL-5)及γ榦擾素(IFN-γ)的錶達.結果 AR組和對照組均有galectin-3和galectin-9蛋白及mRNA的錶達,AR組錶達彊度高于對照組,差異具有統計學意義(t值分彆為11.18、9.261、7.667和6.971,P值均＜0.05).與對照組相比,AR組小鼠外週血中IL-4和IL-5含量均有不同程度的增高(t值分彆為7.202和8.121,P值均＜0.05),而IFN-γ含量則下降(t=-11.94,P＜0.05).galectin 3和galectin 9 mRNA的錶達彊度與外週血中IL-4、IL-5的含量呈正相關(r值分彆為0.667、0.847、0.720及0.736,P值均＜0.05),與IFN-γ含量呈負相關(r值分彆為-0.528和-0.817,P值均＜0.05).結論 galectin 3和galectin 9可能在變應性鼻炎的髮病過程中髮揮一定作用.
목적 탐토소서비점막내반유당응집소3(galectin-3)화반유당응집소9(galectin-9)적표체수평급기여변응성비염적관계.방법 20지BALB/c소서수궤분위변응성비염조(AR조)화대조조,매조10지.AR조분별우제1천화제14천복강주사함란청단백10μg급경양화려2 mg적생리염수현액0.1ml,제21천기비강급여1%란청단백국부격발,지속7 d;대조조용생리염수대체란청단백.통과면역조직화학염색화역전록-취합매련반응(RT-PCR)검측비점막중galectin-3화galectin-9 적표체,동시채용매련면역흡부측정법(ELISA)검측외주혈중자세포개소4(IL-4)、백세포개소5(IL-5)급γ간우소(IFN-γ)적표체.결과 AR조화대조조균유galectin-3화galectin-9단백급mRNA적표체,AR조표체강도고우대조조,차이구유통계학의의(t치분별위11.18、9.261、7.667화6.971,P치균＜0.05).여대조조상비,AR조소서외주혈중IL-4화IL-5함량균유불동정도적증고(t치분별위7.202화8.121,P치균＜0.05),이IFN-γ함량칙하강(t=-11.94,P＜0.05).galectin 3화galectin 9 mRNA적표체강도여외주혈중IL-4、IL-5적함량정정상관(r치분별위0.667、0.847、0.720급0.736,P치균＜0.05),여IFN-γ함량정부상관(r치분별위-0.528화-0.817,P치균＜0.05).결론 galectin 3화galectin 9가능재변응성비염적발병과정중발휘일정작용.
Objective To evaluate the expression of galectin-3 and galeetin-9 in mice nasal mucosa,and to explore the role of galectin-3 and galectin-9 in allergic rhinitis(AR).Methods Twenty mice were randomly divided into AR group and control group,10 mice in each group.Ten mice of BALB/c were sensitized intraperitoneally with 10 μg of ovalbumin(OVA)adsorbed onto 2 mg Al(OH)3 on day 1 and day 14.Mice was induced daily by intranasal daily administration of 10 μl of saline containing 100 μg of OVA from day 21 to day 28.OVA Was replaced with saline in control group.Immunohistochemical staining was used to examine the expression of galectin-3 and galectin-9 in Hasal mucosa.RT-PCR was performed to investigate the level of mRNA expression of complement galectin-3,galectin-9.The level of IL-4,IL-5 and IFN-γ in peripheral blood were titrated by ELISA.Results Galectin-3 and galectin-9 were detected in both groups.Expression of galectin-3 and galectin-9 in group AR was higher than that in control group.Pearson correlation analysis demonstrated that the levels of galectin-3 and galectin-9 were positivly correlaed with the level of IL-4 and IL-5,but negativly correlaed with the level of IFN-γ.Conclusions Galectin-3 and galectin-9 might play an important role in the pathogenesis of allergic rhinitis.
