国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2009年
3期
154-157
,共4页
陈炯%杜敏%杨兵%汤厚阔%翟志敏%李庆
陳炯%杜敏%楊兵%湯厚闊%翟誌敏%李慶
진형%두민%양병%탕후활%적지민%리경
DNT细胞%免疫磁性分离%外周血
DNT細胞%免疫磁性分離%外週血
DNT세포%면역자성분리%외주혈
DNT cells%megnetic activated cell sorting%peripheral blood
目的 建立人DNT细胞的免疫磁性分离(megnetic activated cell sorting,MACS)方法,并检测其在正常人外周血中含量.方法 运用MACS法分离止常人外周血中DNT细胞,用台盼蓝染色流式细胞法检测选出细胞的活性和纯度,运用流式细胞法检测其在正常人外周血中含量.结果 MACS分选法分选出的DNT细胞活性＞97%,纯度为82.77%;DNT细胞占TCRαβ+T细胞的比例(6.25±2.61)%(n=45).结论 MACS法可简单迅速分选出高纯度DNT细胞,且不影响细胞活力.DNT细胞占TCRCD3+T细胞的比例为(6.25±2.61)%.
目的 建立人DNT細胞的免疫磁性分離(megnetic activated cell sorting,MACS)方法,併檢測其在正常人外週血中含量.方法 運用MACS法分離止常人外週血中DNT細胞,用檯盼藍染色流式細胞法檢測選齣細胞的活性和純度,運用流式細胞法檢測其在正常人外週血中含量.結果 MACS分選法分選齣的DNT細胞活性＞97%,純度為82.77%;DNT細胞佔TCRαβ+T細胞的比例(6.25±2.61)%(n=45).結論 MACS法可簡單迅速分選齣高純度DNT細胞,且不影響細胞活力.DNT細胞佔TCRCD3+T細胞的比例為(6.25±2.61)%.
목적 건립인DNT세포적면역자성분리(megnetic activated cell sorting,MACS)방법,병검측기재정상인외주혈중함량.방법 운용MACS법분리지상인외주혈중DNT세포,용태반람염색류식세포법검측선출세포적활성화순도,운용류식세포법검측기재정상인외주혈중함량.결과 MACS분선법분선출적DNT세포활성＞97%,순도위82.77%;DNT세포점TCRαβ+T세포적비례(6.25±2.61)%(n=45).결론 MACS법가간단신속분선출고순도DNT세포,차불영향세포활력.DNT세포점TCRCD3+T세포적비례위(6.25±2.61)%.
Objective The immune magnetic separation(MACS)method of DNT cells was set up and their content Was detected in peripheral blood of healthy man.Methods DNT cells were separated from the peripheral blood.Trypan blue staining and flow cytometry were used to detect their activity and purity,and flow cytometry Was used to detect their content in normal human peripheral blood.Results DNT cell activi ty using MACS sorting was＞97%,the purity Was 82.77%;DNT cells accounted for(6.25±2.61)% (n=45)in the TCRαβ+T cells.Conclusion MACS Can quickly sort out high purity DNT cells,and do not affect the vitality of cells.DNT cells accounted for(6.25±2.61)%in the TCRCD3+T cells.
