国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
4期
317-320
,共4页
汤军%甄利波%钱华%凌晓波%陈建武
湯軍%甄利波%錢華%凌曉波%陳建武
탕군%견리파%전화%릉효파%진건무
清金抗扩饮%支气管扩张症%肿瘤坏死因子-α%中性粒细胞弹性蛋白酶
清金抗擴飲%支氣管擴張癥%腫瘤壞死因子-α%中性粒細胞彈性蛋白酶
청금항확음%지기관확장증%종류배사인자-α%중성립세포탄성단백매
Bronchiectasis%Qingjinkangkuoyin%TNF-α%NE
目的 探讨清金抗扩饮对支气管扩张症(简称“支扩”)模型大鼠的作用及其的疗效机理.方法 建立SD大鼠支扩模型,按随机数字表法随机分为5组,分别为清金抗扩饮高剂量组(灌胃剂量为86.414 g/kg·d-1)、清金抗扩饮低剂量组(灌胃剂量为43.207g/kg·d-1)、左氧氟沙星组(左氧氟沙星液灌胃)、支扩模型组(生理盐水灌胃)、空白对照组(生理盐水灌胃)；治疗2周后观察肺组织病理学特点,外周血中肿瘤坏死因子-α(TNF-α)及炎症细胞水平,肺组织中性粒细胞弹性蛋白酶(NE)水平的变化.结果 清金抗扩饮能显著抑制支扩大鼠血TNF-α水平,高剂量组(137.133±6.1646) ng/L,与支扩模型组(160.425±9.9293) ng/L比较,差异有统计学意义(P＜0.05);血中白细胞水平,高剂量组为(1.106±0.3580) 109/L、低剂量组(1.086±0.2433) 109/L,与支扩模型组(2.017±0.3373) 109/L比较,差异有统计学意义(P＜0.05)；气道扩张程度、肺组织NE水平,高剂量组为(80.697±4.5877) ng/L、低剂量组(80.747±3.6925) ng/L,与支扩模型组(100.040±9.9138) ng/L比较,差异有统计学意义(P＜0.05).结论 清金抗扩饮对支气管扩张症的治疗作用可能通过减轻炎症反应,抑制NE表达实现.
目的 探討清金抗擴飲對支氣管擴張癥(簡稱“支擴”)模型大鼠的作用及其的療效機理.方法 建立SD大鼠支擴模型,按隨機數字錶法隨機分為5組,分彆為清金抗擴飲高劑量組(灌胃劑量為86.414 g/kg·d-1)、清金抗擴飲低劑量組(灌胃劑量為43.207g/kg·d-1)、左氧氟沙星組(左氧氟沙星液灌胃)、支擴模型組(生理鹽水灌胃)、空白對照組(生理鹽水灌胃)；治療2週後觀察肺組織病理學特點,外週血中腫瘤壞死因子-α(TNF-α)及炎癥細胞水平,肺組織中性粒細胞彈性蛋白酶(NE)水平的變化.結果 清金抗擴飲能顯著抑製支擴大鼠血TNF-α水平,高劑量組(137.133±6.1646) ng/L,與支擴模型組(160.425±9.9293) ng/L比較,差異有統計學意義(P＜0.05);血中白細胞水平,高劑量組為(1.106±0.3580) 109/L、低劑量組(1.086±0.2433) 109/L,與支擴模型組(2.017±0.3373) 109/L比較,差異有統計學意義(P＜0.05)；氣道擴張程度、肺組織NE水平,高劑量組為(80.697±4.5877) ng/L、低劑量組(80.747±3.6925) ng/L,與支擴模型組(100.040±9.9138) ng/L比較,差異有統計學意義(P＜0.05).結論 清金抗擴飲對支氣管擴張癥的治療作用可能通過減輕炎癥反應,抑製NE錶達實現.
목적 탐토청금항확음대지기관확장증(간칭“지확”)모형대서적작용급기적료효궤리.방법 건립SD대서지확모형,안수궤수자표법수궤분위5조,분별위청금항확음고제량조(관위제량위86.414 g/kg·d-1)、청금항확음저제량조(관위제량위43.207g/kg·d-1)、좌양불사성조(좌양불사성액관위)、지확모형조(생리염수관위)、공백대조조(생리염수관위)；치료2주후관찰폐조직병이학특점,외주혈중종류배사인자-α(TNF-α)급염증세포수평,폐조직중성립세포탄성단백매(NE)수평적변화.결과 청금항확음능현저억제지확대서혈TNF-α수평,고제량조(137.133±6.1646) ng/L,여지확모형조(160.425±9.9293) ng/L비교,차이유통계학의의(P＜0.05);혈중백세포수평,고제량조위(1.106±0.3580) 109/L、저제량조(1.086±0.2433) 109/L,여지확모형조(2.017±0.3373) 109/L비교,차이유통계학의의(P＜0.05)；기도확장정도、폐조직NE수평,고제량조위(80.697±4.5877) ng/L、저제량조(80.747±3.6925) ng/L,여지확모형조(100.040±9.9138) ng/L비교,차이유통계학의의(P＜0.05).결론 청금항확음대지기관확장증적치료작용가능통과감경염증반응,억제NE표체실현.
Objective To study the effects of Qingjinkangkuoyin (QJKKY) on TNF-α and NE in rats with bronchiectasis.Methods Models were established by intrabronchially injecting with pseudomonas aeruginosa,and divided into 5 groups by random:the QJKKY high dose treatment group (given high dose of QJKKY into stomach),the QJKKY low dose treatment group (given low dose of QJKKY),the levofloxacin group (given levofloxacin),the model group (given normal saline),and the normal contrast group (given normal saline).After 2 weeks of treatment,the histopathology of lung tissue,the levels of TNF-α and inflammatory cells in peripheral blood and NE in rats' lung tissue were detected.Results Compared with the model group (160.425±9.9293)ng/L,QJKKY could decrease the level of TNF-α in blood significantly [high dose of QJKKY treatment group was (137.133±6.1646)ng/L,P＜0.05]; the expression of inflammatory cells in serum were decreased significantly by QJKKY [high dose of QJKKY treatment group was (1.106± 0.3580) 109/L,P＜0.05].Low dose of QJKKY treatment group was (1.086 ±0.2433) 109/L,(P＜0.05) ; the expression of NE in lung tissue were decreased remarkably by QJKKY [high dose of QJKKY treatment group(80.697 ±4.5877)ng/L,P＜0.05]; low dose of QJKKY treatment group is (80.747±3.6925)ng/L,(P＜0.05); and the histopathologic change of lung tissue in QJKKY treatment groups were ameliorated under light microscope by HE staining.Conclusion Qingjinkangkuoyin could cure bronchiectasis by decreasing the expression of TNF-αin peripheral blood and NE in rats' lung tissue.
