CAJ | 학술논문

目的探讨SOCSs基因和JAKs基因在急性髓系白血病(AML)患者中的表达情况.方法 RT-PCR方法检测AML患者和正常对照骨髓SOCS 1～7、JAK 1～3和TYK 2 mRNA的表达.结果 ①AML患者SOCS 1、4、5、7的表达明显低于缓解组和正常对照组(P<0.01),SOCS 3、6表达水平较缓解组和正常对照组高(P<0.01),SOCS 2在各组无明显差异;AML患者JAK2、JAK3、TYK2 mRNA平均表达水平较缓解组和正常对照组明显增高(P<0.05).初治AML患者JAK1 mRNA表达水平较正常对照组略增高,但差异无统计学意义(P>0.05),复发AML患者JAK1 mRNA表达水平较正常对照组明显增高(P<0.05).结论 AML患者的SOCS 1、4、5、7基因表达缺失或降低,JAK家族基因表达明显增高,提示二者可能共同参与髓系白血病的发生.
목적 탐토SOCSs기인화JAKs기인재급성수계백혈병(AML)환자중적표체정황.방법 RT-PCR방법검측AML환자화정상대조골수SOCS 1～7、JAK 1～3화TYK 2 mRNA적표체.결과 ①AML환자SOCS 1、4、5、7적표체명현저우완해조화정상대조조(P<0.01),SOCS 3、6표체수평교완해조화정상대조조고(P<0.01),SOCS 2재각조무명현차이;AML환자JAK2、JAK3、TYK2 mRNA평균표체수평교완해조화정상대조조명현증고(P<0.05).초치AML환자JAK1 mRNA표체수평교정상대조조략증고,단차이무통계학의의(P>0.05),복발AML환자JAK1 mRNA표체수평교정상대조조명현증고(P<0.05).결론 AML환자적SOCS 1、4、5、7기인표체결실혹강저,JAK가족기인표체명현증고,제시이자가능공동삼여수계백혈병적발생.
Objective To investigate the expression of suppressor of cytokine signaling genes (SOCSs) and JAKs mRNA in the acute myloid leukemia(AML) patients. Methods The expression of SOCSs and JAKs mRNA as well as TYK2 in AML patients and healthy adults as normal contrals (NC) was measured with RT-PCR. Results The expression of SOCS 1,4,5 and 7 in AML patients was lower than those in normal control and AML with remis-sion (P<0.01),but the expression of SOCS 3 and 6 was higher than those in normal control and remission AML(P<0.01),however there was no significant difference in SOC2 between groups. The expressions of JAK2 ,JAK3 and TYK2 in AML were significantly higher than those in patients with remission and normal control(P<0.05). The ex-pression of JAK1 mRNA in relapsed AML was higher than that in normal control group(P<0.05),but the latter has no statistical significance between beginning treatment and normal group(P>0.05). Conclusion The deletion and degradion of SOCS 1,4,5 and 7 present in AML patients and JAKs expression is significantly increased, suggesting that both of them may co-participate in the pathogenesis of AML.