山东师大学报(自然科学版)
山東師大學報(自然科學版)
산동사대학보(자연과학판)
JOURNAL OF SHANDONG NORMAL UNIVERSITY
2001年
1期
72-74
,共3页
刘树真%李云龙%孙庆文%毛积芳%石小娟
劉樹真%李雲龍%孫慶文%毛積芳%石小娟
류수진%리운룡%손경문%모적방%석소연
调控序列%地高辛标记%Southern杂交
調控序列%地高辛標記%Southern雜交
조공서렬%지고신표기%Southern잡교
酶切包含20αHSD基因及其调控序列的λ噬菌体DNA，获得4.0kb的DNA大片段，将此片段与载体质粒连接得到重组质粒.酶切重组质粒后电泳，再以地高辛标记的20αHSD基因的cDNA为探针进行Southern杂交.实验获得了大片段的重组质粒.
酶切包含20αHSD基因及其調控序列的λ噬菌體DNA，穫得4.0kb的DNA大片段，將此片段與載體質粒連接得到重組質粒.酶切重組質粒後電泳，再以地高辛標記的20αHSD基因的cDNA為探針進行Southern雜交.實驗穫得瞭大片段的重組質粒.
매절포함20αHSD기인급기조공서렬적λ서균체DNA，획득4.0kb적DNA대편단，장차편단여재체질립련접득도중조질립.매절중조질립후전영，재이지고신표기적20αHSD기인적cDNA위탐침진행Southern잡교.실험획득료대편단적중조질립.
Digesting the λ-phage gene which may carry the promoter region of 20 αHSD, we obtained a 4.0 kb fragment. The fragment was subcloned into pBluescript vector, resulting in a fusion constructs. Digesting the constructs and making southern hybridization with the cDNA of rat 20 αHSD labeled with DIG,we revealed the 4.0 kb fragment having been inserted into 2.9 kb vector using the BamHI site.
