目的 MMP-9和CD44共同定位在细胞表面,且这种共同定位有助于CD44将MMP-9锚定于细胞表面并对胶原Ⅳ降解,促进肿瘤细胞的浸润.用可溶性CD44阻断CD44功能后即可使其丧失锚定MMP-9的作用.而被CD44锚定于细胞表面的MMP-9还可使无活性的TGF-β1前体活化,活化的TGF-β1则是新生血管生长所必需的生长因子.通过对CD44锚定的MMP-9及TGF-β1在脑星形细胞瘤中表达情况进行分析,对其表达意义进行研究,对CD44、MMP-9、TGF-β1在脑星形细胞瘤的浸润转移中的作用进行初步探讨,同时对CD44、MMP-9、TGF-β1相关性进行分析研究.方法 共收集2006年郑州大学第一附属医院和许昌市中心医院手术切除的脑星形细胞瘤标本98例,应用免疫组化SP法检测肿瘤组织中CD44、MMP-9、TGF-β1的表达,分析CD44、MMP-9、TGF-β1和脑星形细胞瘤分级之间以及CD44、MMP-9、TGF-β1之间的关系.结果 (1)CD44在星形细胞瘤组织中表达率77.55%(76/98),显著高于正常腩组织0(P<0.05).通过Spearman等级相关检验,不同级别CD44表达具有统计学意义(r=0.95,P=0.046),星形细胞瘤级别与CD44阳性程度之间呈正相关.用Fishers精确检验法对不同级别进行检验,P=0.00027,说明不同级别之间CD44表达的构成比有差异性.(2)MMP-9在星形细胞瘤组织中表达率69.38%(68/98),显著高于正常脑组织(P<0.05).通过Spearman等级相关检验,不同级别MMP-9表达具有统计学意义(r=0.89,P=0.038).星形细胞瘤级别与MMP-9阳性程度之间呈正相关,用Fishers精确检验法对肿瘤4个级别MMP-9观察结果的构成情况进行检验,P=1.12×10-7.(3)TGF-β1在星形细胞瘤组织中表达率74.481%(73/98),显著高于正常脑组织(P<0.05).通过Spearman等级相关检验,不同级别TGF-β1表达具有统计学意义(r=0.91,P=0.046).星形细胞瘤级别与TGF-β1阳性程度之间呈正相关.用Fishers精确检验法对肿瘤4个级别TGF-β1观察结果的构成情况进行检验P=0.0001.(4)星形细胞瘤中CD44、MMP-9、TGF-β1表达的相关性分析CD44表达阳性的病例中,MMP-9的阳性表达率为94.12%,CD44表达阴性的病例中,MMP-9的阴性表达率为60.00%,CD44的表达和MMP-9表达之间呈正相关关系(列联系数rp=0.496016,P=1.559×1018).CD44表达阳性的病例中,TGF-β1的阳性表达率为82.89%,CD44表达阴性的病例中,TGF-β1的阴性表达率为54.55%,CD44的表达与TGF-β1表达呈正相关关系(列联系数rp=0.3136,P=0.001076).MMP-9表达阳性的病例中,TGF-β1的阳性表达率为91.17%,MMP-9表达阴性的病例中,TGF-β1的阴性表达率为63.33%,经统计学分析表明MMP-9表达与TGF-β1表达呈正相关关系(列联系数rp=0.4825,P=4.93 × 10-8).结论 联合检测MMP-9、CD44、TGF-β1三者对脑星形细胞瘤的预后判断有重要意义.
目的 MMP-9和CD44共同定位在細胞錶麵,且這種共同定位有助于CD44將MMP-9錨定于細胞錶麵併對膠原Ⅳ降解,促進腫瘤細胞的浸潤.用可溶性CD44阻斷CD44功能後即可使其喪失錨定MMP-9的作用.而被CD44錨定于細胞錶麵的MMP-9還可使無活性的TGF-β1前體活化,活化的TGF-β1則是新生血管生長所必需的生長因子.通過對CD44錨定的MMP-9及TGF-β1在腦星形細胞瘤中錶達情況進行分析,對其錶達意義進行研究,對CD44、MMP-9、TGF-β1在腦星形細胞瘤的浸潤轉移中的作用進行初步探討,同時對CD44、MMP-9、TGF-β1相關性進行分析研究.方法 共收集2006年鄭州大學第一附屬醫院和許昌市中心醫院手術切除的腦星形細胞瘤標本98例,應用免疫組化SP法檢測腫瘤組織中CD44、MMP-9、TGF-β1的錶達,分析CD44、MMP-9、TGF-β1和腦星形細胞瘤分級之間以及CD44、MMP-9、TGF-β1之間的關繫.結果 (1)CD44在星形細胞瘤組織中錶達率77.55%(76/98),顯著高于正常腩組織0(P<0.05).通過Spearman等級相關檢驗,不同級彆CD44錶達具有統計學意義(r=0.95,P=0.046),星形細胞瘤級彆與CD44暘性程度之間呈正相關.用Fishers精確檢驗法對不同級彆進行檢驗,P=0.00027,說明不同級彆之間CD44錶達的構成比有差異性.(2)MMP-9在星形細胞瘤組織中錶達率69.38%(68/98),顯著高于正常腦組織(P<0.05).通過Spearman等級相關檢驗,不同級彆MMP-9錶達具有統計學意義(r=0.89,P=0.038).星形細胞瘤級彆與MMP-9暘性程度之間呈正相關,用Fishers精確檢驗法對腫瘤4箇級彆MMP-9觀察結果的構成情況進行檢驗,P=1.12×10-7.(3)TGF-β1在星形細胞瘤組織中錶達率74.481%(73/98),顯著高于正常腦組織(P<0.05).通過Spearman等級相關檢驗,不同級彆TGF-β1錶達具有統計學意義(r=0.91,P=0.046).星形細胞瘤級彆與TGF-β1暘性程度之間呈正相關.用Fishers精確檢驗法對腫瘤4箇級彆TGF-β1觀察結果的構成情況進行檢驗P=0.0001.(4)星形細胞瘤中CD44、MMP-9、TGF-β1錶達的相關性分析CD44錶達暘性的病例中,MMP-9的暘性錶達率為94.12%,CD44錶達陰性的病例中,MMP-9的陰性錶達率為60.00%,CD44的錶達和MMP-9錶達之間呈正相關關繫(列聯繫數rp=0.496016,P=1.559×1018).CD44錶達暘性的病例中,TGF-β1的暘性錶達率為82.89%,CD44錶達陰性的病例中,TGF-β1的陰性錶達率為54.55%,CD44的錶達與TGF-β1錶達呈正相關關繫(列聯繫數rp=0.3136,P=0.001076).MMP-9錶達暘性的病例中,TGF-β1的暘性錶達率為91.17%,MMP-9錶達陰性的病例中,TGF-β1的陰性錶達率為63.33%,經統計學分析錶明MMP-9錶達與TGF-β1錶達呈正相關關繫(列聯繫數rp=0.4825,P=4.93 × 10-8).結論 聯閤檢測MMP-9、CD44、TGF-β1三者對腦星形細胞瘤的預後判斷有重要意義.
목적 MMP-9화CD44공동정위재세포표면,차저충공동정위유조우CD44장MMP-9묘정우세포표면병대효원Ⅳ강해,촉진종류세포적침윤.용가용성CD44조단CD44공능후즉가사기상실묘정MMP-9적작용.이피CD44묘정우세포표면적MMP-9환가사무활성적TGF-β1전체활화,활화적TGF-β1칙시신생혈관생장소필수적생장인자.통과대CD44묘정적MMP-9급TGF-β1재뇌성형세포류중표체정황진행분석,대기표체의의진행연구,대CD44、MMP-9、TGF-β1재뇌성형세포류적침윤전이중적작용진행초보탐토,동시대CD44、MMP-9、TGF-β1상관성진행분석연구.방법 공수집2006년정주대학제일부속의원화허창시중심의원수술절제적뇌성형세포류표본98례,응용면역조화SP법검측종류조직중CD44、MMP-9、TGF-β1적표체,분석CD44、MMP-9、TGF-β1화뇌성형세포류분급지간이급CD44、MMP-9、TGF-β1지간적관계.결과 (1)CD44재성형세포류조직중표체솔77.55%(76/98),현저고우정상남조직0(P<0.05).통과Spearman등급상관검험,불동급별CD44표체구유통계학의의(r=0.95,P=0.046),성형세포류급별여CD44양성정도지간정정상관.용Fishers정학검험법대불동급별진행검험,P=0.00027,설명불동급별지간CD44표체적구성비유차이성.(2)MMP-9재성형세포류조직중표체솔69.38%(68/98),현저고우정상뇌조직(P<0.05).통과Spearman등급상관검험,불동급별MMP-9표체구유통계학의의(r=0.89,P=0.038).성형세포류급별여MMP-9양성정도지간정정상관,용Fishers정학검험법대종류4개급별MMP-9관찰결과적구성정황진행검험,P=1.12×10-7.(3)TGF-β1재성형세포류조직중표체솔74.481%(73/98),현저고우정상뇌조직(P<0.05).통과Spearman등급상관검험,불동급별TGF-β1표체구유통계학의의(r=0.91,P=0.046).성형세포류급별여TGF-β1양성정도지간정정상관.용Fishers정학검험법대종류4개급별TGF-β1관찰결과적구성정황진행검험P=0.0001.(4)성형세포류중CD44、MMP-9、TGF-β1표체적상관성분석CD44표체양성적병례중,MMP-9적양성표체솔위94.12%,CD44표체음성적병례중,MMP-9적음성표체솔위60.00%,CD44적표체화MMP-9표체지간정정상관관계(렬련계수rp=0.496016,P=1.559×1018).CD44표체양성적병례중,TGF-β1적양성표체솔위82.89%,CD44표체음성적병례중,TGF-β1적음성표체솔위54.55%,CD44적표체여TGF-β1표체정정상관관계(렬련계수rp=0.3136,P=0.001076).MMP-9표체양성적병례중,TGF-β1적양성표체솔위91.17%,MMP-9표체음성적병례중,TGF-β1적음성표체솔위63.33%,경통계학분석표명MMP-9표체여TGF-β1표체정정상관관계(렬련계수rp=0.4825,P=4.93 × 10-8).결론 연합검측MMP-9、CD44、TGF-β1삼자대뇌성형세포류적예후판단유중요의의.
Objective MMP-9 and CD44 together position in the cell surface,and this kind of together positions to help the CD44 vto settle anchor roans MMP-9s to also decline a solution to the gum original IV at the cell surface and promote tumor cell gradually. Stopping the empress of the function of CD44 with the dissolubility CD44 can immediately make it lose the function that the anchor man series MMP-9. But is settle by the CD44 anchor manses superficial at the cell of the MMP-9 can also make TGF-β1 of have no activity-TGF-β1 ex- bodies activate and activate of TGF-β1 new-born blood vessel growth is the essential growth factor. This text passes the MMP-9 and TGF-β1 which settles to the CD44 anchor manses-TGF-β1 the expression circumstance carries on analysis in the brain star polygon ceil lump,as to it's express meaning to carry on a research,to the CD44,MMP-9,TGF-β1 gradually transfer amedium function to carry on a first step study in the brain star polygon cell lump,in the meantime to the CD44,MMP-9,TGF-β1 relativities carry on an analytical research. Methods Totally collect 2006 subsidiary hospital of Zheng4 Zhou's university first and Xu3 Chang hospital surgical operation in the city center excision of the brain star polygon coil lump the specimen be 98 and apply an immunity set to turn the SP method examination tumor the CD44,MMP-9,TGF-β1 in the organization-TGF-β1 expression,analytical CD44, MMP-9,TGF-β1 with and CD44, MMP-9,TGF-β1 of the brain star polygon cell lump ratings-TGF- 131. The relation of one its. Results (1)CD44 the expression rote is 77.55%(76/98) in the star polygon cell lump organization and show Zhao Gao to organize 0 at the normal brain (P<0.05). Pass the related examination of the Spearman grade,different Class CD44 expression r=0.95,P=0.046, have covariance to learn meaning,the of the star polygon ceil lump Class and the CD44 male degree-present just related. Use the Fishers precision examination method to carry on an examination to the different Claas,P=0.00027,the composing ratio which explains an of different Class CD44 expressions contains difference. (2)MMP-9 the expression rate is 69.38%(68/98) in the star polygon cell lump organization and show Zhan Gan to organize at the normal brain (P< 0.05). Pass the related examination of the Spearman grade, different Class MMP-9 expression r=0.89,P=0.038,have eovariance to learn meaning.The of the star polygon ceil lump Class and the MMP-9 male degree present just related,the composing circumstance which uses the Fishers precision examination method 4 observation result of the Class MMP-9s to the tumor carries on an examination,P=1.12×10-7. (3)TGF-β1 the expression rate is 74.481%(73/98) in the star polygon ceil lump organization and show Zhao Gao to organize at the normal brain (P<0.05). Pass the related examination of the Spearman grade,different Class TGF-β1 expression r=0.91,P=0.046,have covariance to learn intoning. The star polygon cell lump Class and TGF-β1 of male degrees present just related.Use the Fishers precision examination method 4 Class TGF-β1 to the tumor-TGF-β1 composing circumstance of observation result carry on an examination,P=0.0001. (4)Star polygon cell CD44,MMP-9,TGF in lump-TGF-β1 expression of relativity analysis CD44 expression masculine gender of in the case,the male expression rate of MMP-9 is 94.12%,CD44 expression feminine gender of in the case,the feminine gender expression of the MMP-9 rate is 60.00%, of the expression and the expressions MMP-9s of CD44 presents a just related relation (the row contact few rp=0. 496016, P=1.559 × 10-8). The CD44 expresses a male case In,TGF-β1 male expression rates is 82.89%,in the ease of the CD44 expression feminine gender,TGF-β1 feminine gender expression rates is 54.55%, the expression and TGF-β1 of CD44-TGF-β1 expressions present a just related relation (the row contact few rp=0.3136, P = 0.001076). The MMP-9 expresses a male case in, TGF-β1 male expression rates is 91.17%,in the ease of the MMP-9 expression feminine gender,TGF-β1 feminine gender expression rates is 63.33%,Be learned analysis the enunciation MMP-9 to express by covariance and TGF-β1 expressions present a just related relation (the row contact few rp=0.4825,P=4.93 × 10-8). Conclusion The deteedion of MMP-9,CD44 and TGF-β1 is significance for prognosis of the brain star polygon cell lump.
