CAJ | 학술논문

应用0.5、1.0、2.0、3.0 mg/mL 4种浓度的秋水仙素溶液诱导正在发育的映山红(Rhododendron simsii)、马银花(Rh.ovatum)、满山红(Rh.mariesii)的花芽和叶芽,观察其花粉粒及气孔变异情况.结果表明,3种杜鹃叶芽和花芽表现出的多倍性有一定的变化,映山红花粉粒在3.0 mg/mL的浓度处理时变异最大,增加37.99%;满山红花粉粒在2.0 mg/mL的浓度处理时变异最大,增加21.96%;随着秋水仙素浓度的增加,3种杜鹃的气孔器大小变异增加,在3.0 mg/mL浓度处理时增加最多,出现气孔增长、保卫细胞宽增加、保卫细胞长度增加等多倍体表现;气孔密度变化与多倍性联系规律不明显.
응용0.5、1.0、2.0、3.0 mg/mL 4충농도적추수선소용액유도정재발육적영산홍(Rhododendron simsii)、마은화(Rh.ovatum)、만산홍(Rh.mariesii)적화아화협아,관찰기화분립급기공변이정황.결과표명,3충두견협아화화아표현출적다배성유일정적변화,영산홍화분립재3.0 mg/mL적농도처리시변이최대,증가37.99%;만산홍화분립재2.0 mg/mL적농도처리시변이최대,증가21.96%;수착추수선소농도적증가,3충두견적기공기대소변이증가,재3.0 mg/mL농도처리시증가최다,출현기공증장、보위세포관증가、보위세포장도증가등다배체표현;기공밀도변화여다배성련계규률불명현.
Induction was conducted on developing flower and leaf bud of Rhododendron simsii, R. ovatum and K mariesii with four treatments of colchicines concentrations, 0.5mg/mL, 1.0 mg/mL, 2.0 mg/mL and 3.0 mg/mL. Observation on microspore and stomata of tested leaf and flower buds demonstrated polyploid variation. Microspore of K simsii increased 37.99% with 3.0 mg/mL of colchicines while that of R. mariesii increased 21.96% with 2.0 mg/mL. Stomata of three tested Rhododendrons varied with increase of colchicines concentration topped at 3.0 mg/mL. The variation of stomata density had no evident relation with polyploid.