CAJ | 학술논문

背景:骨髓间充质干细胞具有自身增殖能力强、分化范围广,能够修复损伤的功能组织及免疫调节等功能特点,具有广阔的治疗前景.骨髓间充质干细胞的分离方法众多,在不同的生长时期和不同的生长条件下具宵较大的表达差异,但目前尚无统一的鉴定方法.目的:综述骨髓间充质干细胞的分离方法及其生物学特性,并比较骨髓间充质干细胞在有或无血清,分裂增殖前后,分化和诱导前后的表达差异.方法:应用计算机榆索2003-01/2009-06中国期刊全文数据库(http://dlib.cnki.net/kns50/)相关文章,检索词为"骨髓间充质干细胞,分离,培养,分化,表犁,特点",并限定文章语言种类为中文.同时计算机榆索2003-01/2009-06 Pubmed数据库(http://www.ncbi.nlm.nih.gov/PubMed)相关文章,检索词为"bone marrow mesenchymal stem cells,isolation,culture,induce,marker,characterization",并限定文章语言种类为English.共检索到文献237篇.结果与结论:用全骨髓培养法获得的骨髓间充质干细胞与密度梯度离心法获得的相比,CD44的阳性表达率和CD34的阴性表达率都要低一些.然而,全骨髓培养法的骨髓间充质干细胞比密度梯度离心法的骨髓间充质干细胞具有细胞活性更高,细胞增殖速度更快,细胞汇合时间更早和传代时间更短等优势,至于其他的分离方法存在耗资多、实验设备要求高等缺点.目前对骨髓间充质干细胞的分离晌定主要依靠以下几个条件:贴壁性,细胞表面分子阳性和阴性选择标记,自身增殖能力强,具有多向分化能力.骨髓间充质干细胞在有或无血清,分裂增殖前后,分化和诱导前后具有较大的表达差异.
배경:골수간충질간세포구유자신증식능력강、분화범위엄,능구수복손상적공능조직급면역조절등공능특점,구유엄활적치료전경.골수간충질간세포적분리방법음다,재불동적생장시기화불동적생장조건하구소교대적표체차이,단목전상무통일적감정방법.목적:종술골수간충질간세포적분리방법급기생물학특성,병비교골수간충질간세포재유혹무혈청,분렬증식전후,분화화유도전후적표체차이.방법:응용계산궤유색2003-01/2009-06중국기간전문수거고(http://dlib.cnki.net/kns50/)상관문장,검색사위"골수간충질간세포,분리,배양,분화,표리,특점",병한정문장어언충류위중문.동시계산궤유색2003-01/2009-06 Pubmed수거고(http://www.ncbi.nlm.nih.gov/PubMed)상관문장,검색사위"bone marrow mesenchymal stem cells,isolation,culture,induce,marker,characterization",병한정문장어언충류위English.공검색도문헌237편.결과여결론:용전골수배양법획득적골수간충질간세포여밀도제도리심법획득적상비,CD44적양성표체솔화CD34적음성표체솔도요저일사.연이,전골수배양법적골수간충질간세포비밀도제도리심법적골수간충질간세포구유세포활성경고,세포증식속도경쾌,세포회합시간경조화전대시간경단등우세,지우기타적분리방법존재모자다、실험설비요구고등결점.목전대골수간충질간세포적분리상정주요의고이하궤개조건:첩벽성,세포표면분자양성화음성선택표기,자신증식능력강,구유다향분화능력.골수간충질간세포재유혹무혈청,분렬증식전후,분화화유도전후구유교대적표체차이.
BACKGROUND:Bone marrow mesenchymal stem cells(BMSCs)have the properties involving high proliferation capability,widely distribution,functional tissue repair after injury,as well as immune modulation,by which bring us extensive therapeutic possibilities.There are plenty of methods for isolation of BMSCs,yet,BMSCs exhibit discrepancies in varied growth stage and culture conditions.Up to now,there has been no agreement about the identification methods for cultured BMSCs.OBJECTIVE:To review the isolation methods and biological characteristics of BMSCs,and to compare the differential expression of BMSCs between in serum and serum-free medium,prior to and after proliferation,as well as before and after induction.METHODS:A computer-based online search was performed using key words of "bone marrow mesenchymal stem cells,isolation,culture,induce,marker,and characterization" to find documents published in the database of CNKI (http://dlib.cnki.net/kns50/)or Pubmed(http://www.ncbi.nlm.nih.gov/PubMed)from January 2003 to June 2009.The languages were limited Chinese and English.A total of 237 literatures were searched by the computer.RESULTS AND CONCLUSION:The positive rates of CD44 and CD34 of BMSCs isolated by the whole bone marrow culture were smaller than that of the density gradient centrifugation.However,BMSCs isolated by the whole bone marrow culture were superior to those isolated by the density gradient centrifugation in cell viability,proliferation rate,confluence time,as well as generation time.Other methods for BMSCs isolation had drawbacks of large cost and high requirement of experimental equipments.Following conditions were used to identify BMSCs:cell adherence,cell surface molecule labeling,strong self-proliferation ability,as well as potentials multi-directional differentiation.BMSCs exhibit differential expression between in serum and serum-free medium,prior to and after proliferation,as well as before and after induction.