CAJ | 학술논문

[目的]研究野生型拟南芥叶片愈伤组织的诱导.[方法]以野生型拟南芥叶片为外植体,通过将其切段后接种在添加有不同浓度6-BA和NAA的MS培养基上,研究了拟南芥愈伤组织的诱导和再生植株.[结果]6-BA对于愈伤组织诱导不可缺少,但其浓度太高易产生玻璃化;NAA单独使用利于生根,配合6-BA使用利于分化芽;获得最适愈伤组织诱导培养基为MS+0.50 mg/L 6-BA+0.10 mg/L NAA,愈伤组织诱导率可达100%.[结论]为进一步开展拟南芥的遗传转化或细胞培养奠定了基础.
[목적]연구야생형의남개협편유상조직적유도.[방법]이야생형의남개협편위외식체,통과장기절단후접충재첨가유불동농도6-BA화NAA적MS배양기상,연구료의남개유상조직적유도화재생식주.[결과]6-BA대우유상조직유도불가결소,단기농도태고역산생파리화;NAA단독사용리우생근,배합6-BA사용리우분화아;획득최괄유상조직유도배양기위MS+0.50 mg/L 6-BA+0.10 mg/L NAA,유상조직유도솔가체100%.[결론]위진일보개전의남개적유전전화혹세포배양전정료기출.
[Objective] The callus induction of wild Arabidopsis thaliana leaves was studied. [Method] As explants, leaf segments of wild A. thaliana were inoculated in MS medium including 6-BA and NAA with different concentration, and studying the callus induction and regenera- ted plant of A. thaliana. [Result] 6-BA was necessary for callus induction, but higher concentration resulted into vitrification easily; single use of NAA was beneficial to rooting, and bud differentiation was easy in the medium with NAA and 6-BA; the optimum medium of callus induction was MS +0.5 mg/L 6-BA +0.10 mg/L NAA, with the callus induction frequency of 100%. [Conclusion] Our study could lay a foundation for the ge- netic transformation and cell culture of A. thaliana.